Lentiviral Vectors
Lentiviral Vectors - Related Videos
03:07
Lentiviral Vector-Based Transduction of Rat Fertilized Embryos
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2026
Source: Koza, P., et al. Generation of Transgenic Rats using a Lentiviral Vector Approach. J. Vis. Exp. (2020)This video demonstrates microinjection-assisted delivery of lentiviral vectors into fertilized rat embryos to achieve stable transgene integration for the generation of transgenic rats.
08:46
Production of Lentiviral Vectors for Transducing Cells from the Central Nervous System
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Cited by 44 •
2012
In this protocol we describe production, purification and titration of lentiviral vectors. We provide an example of lentiviral vector-mediated gene delivery in primary cultured neurons and astrocytes. Our methods may also apply to other cell types in vitro and in vivo.
10:42
A Protocol for the Production of Integrase-deficient Lentiviral Vectors for CRISPR/Cas9-mediated Gene Knockout in Dividing Cells
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Cited by 21 •
2017
We describe the production strategy of integrase-deficient lentiviral vectors (IDLVs) as vehicles for delivering CRISPR/Cas9 to cells. With an ability to mediate quick and robust gene editing in cells, IDLVs present a safer and equally effective vector platform for gene delivery compared to integrase-competent vectors.
02:30
Gene Delivery to Mouse Intestinal Organoids Using Lentiviral Vectors
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2026
Source: Van Lidth de Jeude, J. F., et al. Protocol for Lentiviral Transduction and Downstream Analysis of Intestinal Organoids. J. Vis. Exp. (2015)This video describes the lentiviral transduction of mouse intestinal organoids from a crypt-like structure containing suspension. Using a basement membrane matrix as a scaffold and growth factor–supplemented media, lentiviral vectors are introduced to enable stable gene...
16:04
A Tetracycline-regulated Cell Line Produces High-titer Lentiviral Vectors that Specifically Target Dendritic Cells
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Cited by 6 •
2013
Here, we use retroviral transduction and concatemeric transfection to create a cell line that can express the components of a lentiviral vector (LV) in the absence of tetracycline. This LV encodes GFP and is pseudotyped with a glycoprotein, SVGmu, which is specific for a receptor on dendritic cells.
FAQs
After entering a target cell, the vector’s RNA genome is reverse-transcribed into DNA. The enzyme integrase then inserts this DNA into the host-cell genome. Because the introduced genetic information becomes associated with the cell’s genomic material, researchers can examine sustained expression over time in experimental systems designed to study gene function or cellular phenotypes.
Integration enables long-term expression in both dividing and nondividing cells, giving researchers flexibility across different biological systems. This feature supports experiments involving cell types with distinct proliferation behavior, including models used in developmental biology, neuroscience, and regenerative research. It also helps investigators examine introduced genes beyond a brief delivery period.
Lentiviral vectors can carry regulatory sequences in addition to the genetic material being studied. Including these sequences broadens experimental designs by allowing researchers to examine genetic information in a more complete regulatory context. This capability is especially relevant when creating cellular models, investigating gene function, or analyzing how introduced material contributes to a disease-related phenotype.
A typical strategy begins by selecting the target cell and the genetic material to be introduced. Researchers can then incorporate relevant regulatory sequences, deliver the engineered vector to the cells, and rely on reverse transcription followed by genomic integration. Subsequent analysis focuses on stable gene expression, altered cell behavior, or a disease-relevant phenotype.
They are useful when an experiment requires genetic material to remain expressed in target cells over time. Researchers apply them to study gene function, establish cellular models, and produce disease-relevant phenotypes. The same general approach also supports investigations of gene therapies, where sustained expression and access to diverse cell types are important considerations.
Their ability to support stable expression in diverse cell types makes these systems relevant across several areas of biology. Applications include developmental biology, immunology, neuroscience, and regenerative research. In each context, investigators can introduce selected genetic material into cellular models and then examine gene function, resulting phenotypes, or other biology connected to the research question.