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Co-transplantation of Human Ovarian Tissue with Engineered Endothelial Cells: A Cell-based Strategy Combining Accelerated Perfusion with Direct Paracrine Delivery
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Bioengineering
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JoVE Journal Bioengineering
Co-transplantation of Human Ovarian Tissue with Engineered Endothelial Cells: A Cell-based Strategy Combining Accelerated Perfusion with Direct Paracrine Delivery

Co-transplantation of Human Ovarian Tissue with Engineered Endothelial Cells: A Cell-based Strategy Combining Accelerated Perfusion with Direct Paracrine Delivery

DOI:
10.3791/57472-v

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07:40 min

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May 16, 2018

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, , , , , , , ,
1Center for Reproductive Medicine and Infertility,Weill Cornell Medical College, 2Angiocrine Biosciences, Inc., 3Tri-Institutional Stem Cell Derivation Laboratory,Weill Cornell Medical College, 4Joan and Sanford I. Weill Department of Medicine,Weill Cornell Medical College

Chapters

  • 00:04Title
  • 01:06Encapsulation of Ovarian Tissue
  • 03:40Bilateral Oophorectomy and Co-transplantation of Human Ovarian Tissue with Engineered Endothelial Cells into NSG Mice
  • 05:07Results: Co-transplantation of Human Ovarian Tissue with Endothelial Cells
  • 06:57Conclusion

Summary

Automatic Translation

Automatic Translation

For some patients, the only option for fertility preservation is cryopreservation of ovarian tissue. Unfortunately, delayed revascularization undermines follicular viability. Here, we present a protocol to co-transplant human ovarian tissue with endothelial cells for utilization as a cell-based strategy combining accelerated perfusion with a direct paracrine delivery of bioactive molecules.

Tags

Keywords: Co-transplantationEngineered Endothelial CellsCell-based StrategyAccelerated PerfusionParacrine DeliveryXenograftFollicular ActivationFolliculogenesisFibrin ClotAccutaseHemocytometer

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