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Journal / Medicine / 人鼻上皮细胞球体的生成与个体化囊性纤维膜电导调节的研究…
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人鼻上皮细胞球体的生成与个体化囊性纤维膜电导调节的研究
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人鼻上皮细胞球体的生成与个体化囊性纤维膜电导调节的研究

Article DOI: 10.3791/57492-v • 08:00 min • April 11th, 2018
April 11th, 2018 •
1, 1, 1, 1, 1, 1, 1
1Department of Pediatrics, Division of Pulmonary Medicine, Cincinnati Children's Hospital Medical Center

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这里我们描述了一种生成三维的人鼻上皮细胞的球形培养方法。球体被刺激以驱动囊性纤维化跨膜电导调节剂 (CFTR) 依赖的离子和液体分泌物, 将球体腔内大小的变化量化为 CFTR 功能的代表。

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医学 问题 134 囊性纤维化 CFTR 精密医学 Organoid 球形 鼻腔 细胞培养
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