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Microinjection of Western Corn Rootworm, Diabrotica virgifera virgifera, Embryos for Germline Transformation, or CRISPR/Cas9 Genome Editing
JoVE Journal
Bioengineering
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JoVE Journal Bioengineering
Microinjection of Western Corn Rootworm, Diabrotica virgifera virgifera, Embryos for Germline Transformation, or CRISPR/Cas9 Genome Editing

Microinjection of Western Corn Rootworm, Diabrotica virgifera virgifera, Embryos for Germline Transformation, or CRISPR/Cas9 Genome Editing

DOI:
10.3791/57497-v

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07:42 min

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April 27, 2018

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, , , ,
1Department of Entomology and Plant Pathology,North Carolina State University

Chapters

  • 00:05Title
  • 00:51Setting Up Colonies of WCR Adults
  • 02:02Preparing for Microinjection
  • 04:24Microinjection and Post-injection Care
  • 05:41Results: First Transformation Attempts
  • 06:54Conclusion

Summary

Automatic Translation

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Here we present protocols for collecting and microinjecting precellular western corn rootworm embryos for the purpose of performing functional-genomic assays such as germline transformation and CRISPR/Cas9-genome editing.

Tags

Western Corn RootwormDiabrotica Virgifera VirgiferaMicroinjectionGermline TransformationCRISPR/Cas9Genome EditingEmbryoRearingArtificial DietEgg CollectionInsect Incubator

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