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Journal / Bioengineering / Microinjection of Western Corn Rootworm,…
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Bioengineering

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JoVE Journal Bioengineering
Microinjection of Western Corn Rootworm, Diabrotica virgifera virgifera, Embryos for Germline Transformation, or CRISPR/Cas9 Genome Editing
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Microinjection of Western Corn Rootworm, Diabrotica virgifera virgifera, Embryos for Germline Transformation, or CRISPR/Cas9 Genome Editing

Article DOI: 10.3791/57497-v • 07:42 min • April 27th, 2018
April 27th, 2018 •
1, 1, 1, 1, 1
1Department of Entomology and Plant Pathology, North Carolina State University

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Summary
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Here we present protocols for collecting and microinjecting precellular western corn rootworm embryos for the purpose of performing functional-genomic assays such as germline transformation and CRISPR/Cas9-genome editing.

Tags

Microinjection Western Corn Rootworm Diabrotica Virgifera Virgifera Germline Transformation CRISPR/Cas9 Genome Editing Pest Control Methods BT Toxin RANI Southern Rootworms Northern Rootworms Injection-based RANI Laboratory Rearing Survival Rates Rootworm Adults Artificial Diet Petri Dishes Water Reservoir
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