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Identifying Transcription Factor Olig2 Genomic Binding Sites in Acutely Purified PDGFRα+ Cells by Low-cell Chromatin Immunoprecipitation Sequencing Analysis
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JoVE Journal Neuroscience
Identifying Transcription Factor Olig2 Genomic Binding Sites in Acutely Purified PDGFRα+ Cells by Low-cell Chromatin Immunoprecipitation Sequencing Analysis

Identifying Transcription Factor Olig2 Genomic Binding Sites in Acutely Purified PDGFRα+ Cells by Low-cell Chromatin Immunoprecipitation Sequencing Analysis

DOI:
10.3791/57547-v

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12:29 min

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April 16, 2018

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, , ,
1The Vivian L. Smith Department of Neurosurgery, Center for Stem Cell and Regenerative Medicine,University of Texas Health Science Center

Chapters

  • 00:04Title
  • 01:10Purification of PDGFRα Positive Oligodendrocyte Lineage Cells from Mouse
  • 05:03Low-cell ChIP Preparation and ChIP Library Construction for High-throughput Sequencing
  • 09:41Results: Use of ChIP Analysis to Study Genome-wide Binding of the Olig2 in OPCs
  • 11:42Conclusion

Summary

Automatic Translation

Automatic Translation

Here we present a protocol which is designed to analyze the genome-wide binding of the oligodendrocyte transcription factor 2 (Olig2) in acutely purified brain oligodendrocyte precursor cells (OPCs) by performing low-cell chromatin immunoprecipitation (ChIP), library preparation, high-throughput sequencing and bioinformatic data analysis.

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Keywords: Transcription Factor Olig2PDGFR-α+ CellsChIP-seqLow-cell ChIP-seqOligodendrocyte Precursor CellsImmunopanningGenome-wide Transcriptional RegulationEpigenetic MechanismsCell DifferentiationCell Development

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