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Journal / Neuroscience / Recording and Modulation of Epileptiform Activity …
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Neuroscience

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JoVE Journal Neuroscience
Recording and Modulation of Epileptiform Activity in Rodent Brain Slices Coupled to Microelectrode Arrays
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Recording and Modulation of Epileptiform Activity in Rodent Brain Slices Coupled to Microelectrode Arrays

Article DOI: 10.3791/57548-v • 10:24 min • May 15th, 2018
May 15th, 2018 •
1, 1, 1,2
1Department of Neuroscience and Brain Technologies, Istituto Italiano di Tecnologia, 2Rehab Technologies, Istituto Italiano di Tecnologia

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Summary
Automatic Translation

We illustrate how to perform recording and electrical modulation of 4-aminopyridine-induced epileptiform activity in rodent brain slices using microelectrode arrays. A custom recording chamber maintains tissue viability throughout prolonged experimental sessions. Live electrode mapping and selection of stimulating pairs are performed by a custom graphical user interface.

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Recording Modulation Epileptiform Activity Rodent Brain Slices Microelectrode Arrays Neuromodulation Epilepsy Treatment Brain Area Stimulation Protocol Advantage Technique Rodent Brain Circuits Observation Points Inter-electrode Distance MEA (microelectrode Array) Elastomeric Sealant Recording Chamber Bubbles Tweezers Sealant Layer Moist Environment Cure At Room Temperature Overnight Recording Bench Warm Bath Holding ACSF (artificial Cerebrospinal Fluid) Pre-warming Chambers Oxygen And Carbon Dioxide Mixture Inverted Pasteur Pipette Room Temperature Holding Chamber Hippocampus-cortex Brain Slices Recovery Period
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