Name: The Perinatal Asphyxiated Lamb Model: A Model for Newborn Resuscitation (Video) | JoVE Journal
Uploaded: 2018-08-15T12:30:00.000+00:00
Duration: 9 min 3 s
Description: Learn the perinatal asphyxiated lamb model for studying neonatal resuscitation and birth asphyxia using invasive instrumentation in fetal lambs. This method enables precise physiological measurements during fetal to neonatal transition.

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5 minutes

All protocols have been approved by the Institutional Animal Care and Use Committee (IACUC) by the State University of New York Buffalo. An illustration of the methodology depicting invasive and non-invasive monitoring is shown in Figure 1.
1. Animals
<ol>
	<li>Use time-dated, Q-fever seronegative, pregnant ewes (mixed-breed, Suffolk-Dorset-Katahdin breed) with fetal lambs at 127&#8211;143 days gestation. 
	NOTE: Sheep term gestation is 145 days and 127 gestation lambs behave like extreme premature infants.</li>
</ol>
2. Pre-surgical Preparation
<ol>
	<li>Withhold food from the ewe 12 h prior to the surgery.</li>
	<li>Sedate the ewe with intravenous (IV) diazepam (0.25&#8211;1.5 mg/kg) and ketamine (4 mg/kg).</li>
	<li>Guide the ewe into lateral recumbency on to a lift cart.</li>
	<li>Move the ewe to dorsal recumbency onto a V-top surgical table.</li>
</ol>
3. Anesthesia
NOTE: Prior to the caesarean section, the ewe is sedated with diazepam and ketamine and intubated followed by continuous inhalation of isoflurane (1&#8211;4%). Adequacy of anesthesia is monitored by evaluation of muscle tone and eye reflexes along with continuous monitoring of blood oxygen level with a pulse oximeter and the heart rate. During the instrumentation, lambs will be under the influence of maternal anesthesia and will also receive local anesthetic to sites of instrumentation.
<ol>
	<li>Intubate the ewe with a 10.0 mm cuffed endotracheal tube (ETT). Advance the ETT through the vocal cords 1&#8211;2 cm beyond the cuff. Inflate the ETT cuff and tie the ETT around the ewe&#39;s jaw to secure it in place.
	<ol>
		<li>When the palpebral response is absent, adjust the isoflurane (2&#8211;3% is usually sufficient).</li>
		<li>Provide artificial breaths by conventional mechanical ventilation (CMV) with a fraction of inspired oxygen (FIO2) of 0.21. Set the ventilator to provide tidal volumes of 10&#8211;15 mL/kg.</li>
		<li>Monitor the oxyhemoglobin saturation continuously by a pulse oximeter placed on the ewe&#39;s tongue.</li>
		<li>Measure the end-tidal carbon dioxide (EtCO2) with a capnograph.</li>
		<li>Adjust the FIO2 and CMV to maintain oxyhemoglobin saturation between 90 - 97% and exhaled CO2 between 35&#8211;45 mm Hg, respectively.</li>
	</ol>
	</li>
	<li>Secure the ewe&#39;s limbs to the surgical table with surgical tie-downs.
	<ol>
		<li>Adjust the surgical table to reverse Trendelenburg position to relieve pressure on the fetus.</li>
	</ol>
	</li>
	<li>Place an orogastric tube to decompress and drain gastric contents.</li>
	<li>Supplement IV fluid (normal saline or Lactated Ringers solution) at 10 mL/kg/h.</li>
	<li>Place a rectal temperature probe to obtain continuous core temperature.</li>
</ol>
4. Surgery
NOTE: The cesarean section and fetal procedures are considered acute surgeries in which the animals are euthanized by administering sodium pentobarbital 100 mg/kg IV. Ewes are euthanized following the delivery of the lambs and the lambs are euthanized after the completion of the experiments. Euthanasia is confirmed by asystole. A secondary method of bilateral thoracotomy or exsanguination is also employed. In this case, the aseptic technique is not practiced during the surgery. Personnel still need to wear protective attire during animal contact.
<ol>
	<li>Shave the wool from the ventral abdomen.</li>
	<li>Use the cautery to make a 15&#8211;18 cm abdominal skin-deep incision over the linea alba to expose the fascia.
	<ol>
		<li>Create a small opening into the abdomen using pressure with a blunt tipped hemostat.</li>
		<li>Maintain this opening while sliding a blunt tipped scissor into the abdominal wall.</li>
		<li>Use the scissors to complete the incision of the linea alba.</li>
	</ol>
	</li>
	<li>Locate and exteriorize the fetal head within the uterus from the abdomen.
	<ol>
		<li>Hold the fetal head with one hand and use the cautery to make a 10 cm incision through the uterine wall (over the lamb&#39;s forehead). Ensure to avoid any cotyledons.</li>
		<li>Use Babcock forceps to clamp the layers of the uterus and placenta together at four opposing corners.</li>
		<li>Expose the lamb&#39;s head through the uterus and outside of the ewe&#39;s abdomen.</li>
		<li>Leave the Babcock forceps laying outside of the abdomen during the fetal surgery.</li>
	</ol>
	</li>
</ol>
5. Fetal Airway
<ol>
	<li>Intubate the partially exposed fetal lamb with a 3.5&#8211;4.5 mm cuffed ETT. Advance the ETT through the vocal cords 1 cm beyond the cuff.
	<ol>
		<li>Inflate the cuff and secure the ETT by tying an umbilical tape around the tube and then around the head.</li>
		<li>Allow excess fetal lung fluid in the ETT to drain passively by tilting the head to the side. This simulates egress of lung liquid during labor.</li>
		<li>Occlude the ETT to prevent gas exchange during gasping in the asphyxial period.</li>
	</ol>
	</li>
</ol>
6. Neck Vessel Instrumentation
<ol>
	<li>Shave the wool and infiltrate 1&#8211;2 mL of subcutaneous bupivacaine hydrochloride (0.25%) at all incision sites.
	<ol>
		<li>Make two 3 cm skin incisions on each side of the neck (approximately 6 cm distal to the head) adjacent to the trachea. The incisions should transverse the neck.</li>
		<li>Perform the incision using the cautery while tenting the skin, to avoid lacerating the deep tissue.</li>
	</ol>
	</li>
	<li>Separate the fascia using curved Kelly or mosquito forceps.</li>
	<li>Isolate the right internal jugular vein and right common carotid artery.
	<ol>
		<li>Place two 20 cm, 0 silk sutures under both vessels.</li>
		<li>Allow a (proximal to distal) space of 1 cm between the silk sutures.</li>
		<li>Gently lift each suture that is around the vessel, superficially, to restrict blood flow and make a 1&#8211;2 mm transverse cut into the vessel using Iris surgical scissors.</li>
	</ol>
	</li>
	<li>For the carotid artery, insert one pre-flushed&#160;catheter (15&#8211;17 G) in the right carotid artery caudally towards the aortic arch for blood pressure monitoring and blood sampling.
	<ol>
		<li>Use the proximal tie to ligate the artery completely.</li>
		<li>Repeat steps 6.3.2&#160;- 6.3.3.</li>
		<li>Insert the pre-flushed carotid arterial catheter and use the distal silk suture to tie around both the artery and catheter with 3 knots.</li>
	</ol>
	</li>
	<li>Use a similar technique, to insert one pre-flushed catheter (14&#8211;16 G) into the jugular vein, repeat steps 6.3.2&#8211;6.3.3, advance 7&#8211;10 cm caudally to rest within the thoracic inlet (towards the right atrium). This is used for fluid and medication administration as well as venous pressure measurements.
	<ol>
		<li>Check the catheter for patency with heparinized saline and then complete the suture tie around the vessel and catheter (with the proximal suture only) using&#160;2&#8211;3 knots.</li>
		<li>Insert the second catheter (14&#8211;16 G) approximately 5 cm rostrally to collect blood from the cerebral circulation. Use the same cut for the placement of both catheters.</li>
		<li>Repeat step 6.5.1 as it pertains to the distal suture and catheter and then tie the silk sutures around the opposing catheter lines to secure them in place to prevent twisting.</li>
	</ol>
	</li>
	<li>Extend the previously made incision into a T-shape (1&#8211;2 cm) on the left side.
	<ol>
		<li>Elevate the left carotid artery using opened curved hemostats.</li>
		<li>Place a 2 mm perivascular flow-probe around the vessel to measure the blood flow.</li>
		<li>Cover the flow-probe with a 1-cm flexible polymer sleeve to stabilize the probe in position.</li>
		<li>Place the flow-probe cable line into the T-shaped incision, allowing the cable to run parallel with the vessel.</li>
		<li>Close the skin incision. Tie around the cable, and around a loop of the cable, to avoid restricting the flow if pulled.</li>
	</ol>
	</li>
</ol>
7. Great Vessel Instrumentation
<ol>
	<li>Extract the fetal lamb to expose the chest, and cover the exposed skin with polyethylene wrap to prevent heat loss.</li>
	<li>Place the lamb in right lateral recumbency.</li>
	<li>Position the left forelimb around the neck to expose the surgical site.</li>
	<li>Infiltrate 3 mL of bupivacaine hydrochloride (0.25%) along the 4th intercostal space and make a 6 cm skin incision using the cautery.
	<ol>
		<li>Carefully pierce the intercostal muscle with blunt curved forceps, circle under the 3rd rib, and come out between the next intercostal space. Make sure not to injure the lung or the heart.</li>
		<li>Open the clamp for a pre-cut piece of 0 silk and bring it through to encircle the rib.</li>
		<li>Repeat steps 7.4.1&#8211;7.4.2 to secure the suture under the 4th rib.</li>
		<li>Clamp the ties around each rib. Gently pull up and insert a cotton-tipped applicator into the chest under the 4th intercostal space.</li>
		<li>Use the applicator stick to protect the chest contents while using the cautery to open the intercostal space in 1 cm for a final opening of 6 - 8 cm. Ensure that the lung is not caught in the rib ties.</li>
		<li>Place a rib spreader into the chest-opening. Opening the chest will reveal the left upper lobes of the lung, the main pulmonary artery (PA) and the ductus arteriosus.</li>
	</ol>
	</li>
	<li>Use 2 x 2 inch gauzes to envelop the lung and gently push any exposed tissue away from the surgical area.
	<ol>
		<li>Use a small manual retractor to improve the field of view (if necessary).</li>
		<li>Place an umbilical tape (1/4 inch width, 6 inches length) pre-soaked in amnion around the great vessels. Soaking the umbilical tape decreases friction (and injury) onto the vessel walls.</li>
	</ol>
	</li>
	<li>Using forceps, lift the pericardium and cut along the main pulmonary artery, taking care not to cut the vagal nerve. Use an applicator stick to keep the right atrium from interfering with a lateral cut of the pericardium along the vagal nerve.</li>
	<li>Using Gemini forceps, move the forceps slowly from the ductal/PA notch around and behind the left PA to &#34;encircle&#34; and emerge on the other side of the left PA. Many delicate tissues run under this area and proceed only if there is no resistance.
	<ol>
		<li>When the tip of the forceps is observed, open only far enough to grab one end of the pre-moistened umbilical tape. Clamp with only 1 position clicked in place. It is possible that surrounding tissue could be caught in the forceps too, and if tightly clamped that tissue might be caught with the umbilical tape resulting in vessel injury.</li>
		<li>Gently back out the forceps to pull the tape into position. This serves to open a space for the flow probe and helps to direct the insertion of the flow probes (Figure 2). Care to protect the notch portion of any vessel is imperative as this is the most fragile part of the tissue.</li>
	</ol>
	</li>
	<li>Place a 4&#8211;6 mm perivascular flow-probe by gently lifting the umbilical tape and guiding the (L-bracket) metal portion of the probe around the vessel along the established opening.
	<ol>
		<li>Direct the tape toward the back of the probe to help seat the vessel in place and to visualize the end of the L-bracket thus allowing the sliding closure to be secured.</li>
		<li>Make sure the vessel is &#62; 75% of the probe size to ensure a tight fit and accurate flow measurements.</li>
		<li>Carefully cut the end of the umbilical tape close to the probe and gently pull the other end to remove the tape from the vessel. The tape will interfere with measurements if not removed.</li>
		<li>Apply ultrasound gel and adjust the probe and cable position to improve signal intensity and quality.</li>
	</ol>
	</li>
	<li>Repeat steps 7.6&#8211;7.7.4 as it pertains to the placement of a 6 - 8 mm perivascular flow-probe around the ductus arteriosus.</li>
	<li>Remove any protective 2 x 2 gauze from the chest and allow the lung to re-position.</li>
	<li>Place a new 2 x 2 gauze around each metal bracket of the flow probe to protect the lung.</li>
	<li>Close the chest in layers using a tapered needle and 2.0 synthetic monofilament suture. Secure the cables of the flow-probes to the outer skin with a loop using a cutting needle and 2.0 silk suture.</li>
</ol>
8. Non-invasive Measurement
<ol>
	<li>Attach EKG leads at the right axilla, left axilla, and right inguinal area (three-lead EKG). Pre- and post-ductal arterial oxyhemoglobin saturation (SpO2) is monitored with a pulse oximeter placed on the right forelimb and either hindlimb, respectively. Secure a near-infrared spectroscope (NIRS) monitor over the forehead with sutures and an opaque dressing.</li>
</ol>
9. Data Collection
<ol>
	<li>Collect and record data using a data acquisition software. Figure 1 illustrates invasive and non-invasive parameters including: arterial and central venous blood pressure, left common carotid, left pulmonary and ductal arterial blood flow, EKG, SpO2, NIRS, ETCO2. 
	NOTE: The software and equipment set-up for data collection can vary and is beyond the scope of this article.</li>
</ol>
10. Experimental Protocol
<ol>
	<li>Occlude and cut the umbilical cord, and move the lamb from the maternal abdomen to a radiant warmer.</li>
	<li>During the asphyxial period, insert an umbilical venous catheter for epinephrine administration (0.01&#8211;0.03 mg/kg IV, per NRP recommendations).</li>
	<li>Insert an umbilical arterial catheter to collect post-ductal arterial blood gases.</li>
	<li>Monitor the lamb for impending asystole, which can be defined as the absence of detected carotid flow and pressure.</li>
	<li>Ensure that adequate personnel is available and dedicate each person to the following tasks: (1) manage ventilation and the airway, (2) perform chest compressions, (3) administer medication, (4) draw blood samples, (5) lead the code.</li>
	<li>Begin resuscitation by providing positive pressure ventilation (PPV) with a T-piece resuscitator at pressures of 35/5 cm H2O.&#160;Initiate the 20 min timer for experimental resuscitation protocol.</li>
	<li>After 30 s of ventilation, begin chest compressions by placing the thumb and fingers of one hand on opposite sides of the chest.
	<ol>
		<li>Provide chest compressions by squeezing the fingers and thumb together to a depth of approximately 1/3.</li>
	</ol>
	</li>
	<li>Administer epinephrine at 3-min intervals.</li>
	<li>Continue resuscitation efforts until return of spontaneous circulation or up to 20 min.</li>
	<li>At return of spontaneous circulation, continuous IV fluids (5 cc/kg/hr) and fentanyl (0.5-2.0 mcg/kg/hr; titrated to effect), are given through the previously established jugular venous catheter. Following the 20 min resuscitation period, the animal is euthanized. If lambs do not achieve ROSC by 20 min, proceed with euthanasia.&#160;</li>
</ol>

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<li>Morin, F. C., Egan, E. A., Ferguson, W., Lundgren, C. E. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Development+of+pulmonary+vascular+response+to+oxygen%5BTitle%5D)+AND+%22Am+J+Physiol%22%5BJournal%5D)">Development of pulmonary vascular response to oxygen.</a> Am J Physiol. 254 (3 Pt 2), H542-H546 (1988).</li>
<li>Rawat, M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Neonatal+resuscitation+adhering+to+oxygen+saturation+guidelines+in+asphyxiated+lambs+with+meconium+aspiration%5BTitle%5D)+AND+%22Pediatr+Res%22%5BJournal%5D)">Neonatal resuscitation adhering to oxygen saturation guidelines in asphyxiated lambs with meconium aspiration.</a> Pediatr Res. , (2015).</li>
<li>Chandrasekharan, P. K., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Continuous+End-Tidal+Carbon+Dioxide+Monitoring+during+Resuscitation+of+Asphyxiated+Term+Lambs%5BTitle%5D)+AND+%22Neonatology%22%5BJournal%5D)">Continuous End-Tidal Carbon Dioxide Monitoring during Resuscitation of Asphyxiated Term Lambs.</a> Neonatology. 109 (4), 265-273 (2016).</li>
<li>Lakshminrusimha, S., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Tracheal+suctioning+improves+gas+exchange+but+not+hemodynamics+in+asphyxiated+lambs+with+meconium+aspiration%5BTitle%5D)+AND+%22Pediatr+Res%22%5BJournal%5D)">Tracheal suctioning improves gas exchange but not hemodynamics in asphyxiated lambs with meconium aspiration.</a> Pediatr Res. 77 (2), 347-355 (2015).</li>
<li>Meuli, M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((In+utero+surgery+rescues+neurological+function+at+birth+in+sheep+with+spina+bifida%5BTitle%5D)+AND+%22Nat+Med%22%5BJournal%5D)">In utero surgery rescues neurological function at birth in sheep with spina bifida.</a> Nat Med. 1 (4), 342-347 (1995).</li>
<li>Meuli, M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Creation+of+myelomeningocele+in+utero%3A+a+model+of+functional+damage+from+spinal+cord+exposure+in+fetal+sheep%5BTitle%5D)+AND+%22J+Pediatr+Surg%22%5BJournal%5D)">Creation of myelomeningocele in utero: a model of functional damage from spinal cord exposure in fetal sheep.</a> J Pediatr Surg. 30 (7), 1028-1032 (1995).</li>
<li>Adzick, N. S., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Correction+of+congenital+diaphragmatic+hernia+in+utero.+IV.+An+early+gestational+fetal+lamb+model+for+pulmonary+vascular+morphometric+analysis%5BTitle%5D)+AND+%22J+Pediatr+Surg%22%5BJournal%5D)">Correction of congenital diaphragmatic hernia in utero. IV. An early gestational fetal lamb model for pulmonary vascular morphometric analysis.</a> J Pediatr Surg. 20 (6), 673-680 (1985).</li>
<li>Harrison, M. R., Bressack, M. A., Churg, A. M., de Lorimier, A. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Correction+of+congenital+diaphragmatic+hernia+in+utero.+II.+Simulated+correction+permits+fetal+lung+growth+with+survival+at+birth%5BTitle%5D)+AND+%22Surgery%22%5BJournal%5D)">Correction of congenital diaphragmatic hernia in utero. II. Simulated correction permits fetal lung growth with survival at birth.</a> Surgery. 88 (2), 260-268 (1980).</li>
<li>Turley, K., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Intrauterine+cardiothoracic+surgery%3A+the+fetal+lamb+model%5BTitle%5D)+AND+%22Ann+Thorac+Surg%22%5BJournal%5D)">Intrauterine cardiothoracic surgery: the fetal lamb model.</a> Ann Thorac Surg. 34 (4), 422-426 (1982).</li>
<li>Reddy, V. M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((In+utero+placement+of+aortopulmonary+shunts.+A+model+of+postnatal+pulmonary+hypertension+with+increased+pulmonary+blood+flow+in+lambs%5BTitle%5D)+AND+%22Circulation%22%5BJournal%5D)">In utero placement of aortopulmonary shunts. A model of postnatal pulmonary hypertension with increased pulmonary blood flow in lambs.</a> Circulation. 92 (3), 606-613 (1995).</li>
<li>Hemway, R. J., Christman, C., Perlman, J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((The+3%3A1+is+superior+to+a+15%3A2+ratio+in+a+newborn+manikin+model+in+terms+of+quality+of+chest+compressions+and+number+of+ventilations%5BTitle%5D)+AND+%22Arch+Dis+Child+Fetal+Neonatal+Ed%22%5BJournal%5D)">The 3:1 is superior to a 15:2 ratio in a newborn manikin model in terms of quality of chest compressions and number of ventilations.</a> Arch Dis Child Fetal Neonatal Ed. 98 (1), F42-F45 (2013).</li>
<li>Solevåg, A. L., Schmölzer, G. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Optimal+Chest+Compression+Rate+and+Compression+to+Ventilation+Ratio+in+Delivery+Room+Resuscitation%3A+Evidence+from+Newborn+Piglets+and+Neonatal+Manikins%5BTitle%5D)+AND+%22Frontiers+in+Pediatrics%22%5BJournal%5D)">Optimal Chest Compression Rate and Compression to Ventilation Ratio in Delivery Room Resuscitation: Evidence from Newborn Piglets and Neonatal Manikins.</a> Frontiers in Pediatrics. 5 (3), (2017).</li>
<li>Solevåg, A. L., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Chest+compressions+in+newborn+animal+models%3A+A+review%5BTitle%5D)+AND+%22Resuscitation%22%5BJournal%5D)">Chest compressions in newborn animal models: A review.</a> Resuscitation. 96, 151-155 (2015).</li>
</ol>

The authors have no conflicts of interest to declare

The lamb model is comparable in size to human newborns and allows easy instrumentation to measure invasive hemodynamic variables. The fetal/newborn lamb model has been an invaluable research tool that has richly contributed to understanding the transitioning circulation, as well as the newborn&#39;s pulmonary and cardiovascular physiology. Several experimental lamb models have been established over the years to study optimal ventilation strategies in preterm lambs13,14...

Perinatal asphyxia occurs in roughly 4 per 1,000 term births in the United States and is responsible for approximately 25% of the 4 million neonatal deaths worldwide1,2. Throughout the fetus&#39;s natural development, several adaptations must take place during labor and at birth to allow for a seamless transition from the intra- to the extrauterine environment when the lungs take on the role of the placenta as the organ of gas exchange. Any failure of the newborn to adequately transition at birth further compromises resuscitative efforts. Instances when fetal lung clearance is incomplete or delayed3,4, and circumstances that result in a persistent high pulmonary vascular resistance (PVR)5 impact the efficacy of ventilation, which remains the most important intervention in the resuscitation of the asphyxiated newborn6. In addition, immediate clamping of the umbilical cord and removal of the low-resistance placenta can lead to abrupt changes in cardiac output that may cause myocardial dysfunction7,8.
Owing to the infrequent need for aggressive resuscitation (need for chest compressions and/or epinephrine administration)1,9, there is a lack of strong evidence from large randomized clinical trials to support the current neonatal resuscitation program (NRP) guidelines. Many translational research studies in neonatal resuscitation are conducted using postnatal animal models (particularly piglets) that fail to adequately depict the transitioning fetal circulation and fluid-filled lungs inherent to the newborn in the delivery room. Given the unique challenges related to transition from fetal circulation to neonatal circulation, the perinatal asphyxiated cardiac arrest fetal lamb model is ideal to study newborn resuscitative physiology.
The studies by Joseph Barcroft on fetal lambs, as early as the 1930&#39;s, laid the foundation for fetal and neonatal physiology10. In the second half of the 20th century, Geoffrey Dawes&#39; innovative and meticulous experiments on fetal lamb models, and later those by Abraham Rudolph have tremendously contributed to the knowledge of cardiovascular and pulmonary physiology in the fetus11,12. In recent years, studies on fetal/neonatal lamb models have provided a better understanding of the impact of ventilation on hemodynamics13,14, the effects of oxygenation on PVR15,16, as well as the circulatory changes that occur during cord clamping7,17. Finally, in the past year, the newborn lamb has served as a novel model to study the hemodynamic effects during resuscitation18,19,20. A step-by-step narrative of what is involved in conducting a lamb experiment, as well as a detailed description of the surgical instrumentations and the experimental methodology will be presented.

<table><tbody><tr><td>Babcock forceps</td><td>Miltex</td><td>16-44</td><td></td></tr><tr><td>Blood pressure transducer</td><td>Becton Dickinson</td><td>P23XL-1</td><td>Used with saline filled diaphragm domes</td></tr><tr><td>Blunt tipped scissors</td><td>Miltex</td><td>98SCS50-56</td><td></td></tr><tr><td>Capnograph</td><td>Philips</td><td>7900</td><td>Used with Neonatal Flow Sensors</td></tr><tr><td>Cautery pencil</td><td>Valley Lab</td><td>287879</td><td></td></tr><tr><td>Cautery unit</td><td>Valley Lab</td><td>SSE2K</td><td></td></tr><tr><td>Curved Forceps</td><td>Everost</td><td>711714</td><td></td></tr><tr><td>Data acquisition software</td><td>Biopac Systems Inc.</td><td>ACK100W</td><td></td></tr><tr><td>EKG</td><td>Biopac Systems Inc.</td><td>ECG100C</td><td></td></tr><tr><td>Endotracheal tube -cuffed</td><td>Rusch</td><td>111780035</td><td></td></tr><tr><td>Flow modulator</td><td>Transonic Systems Inc.&amp;nbsp;</td><td>T403</td><td></td></tr><tr><td>Flow-probe</td><td>Transonic Systems Inc.&amp;nbsp;</td><td>MC4PSS-LS-WC100-CM4B-GA</td><td></td></tr><tr><td>Gastric tube</td><td>Jorgensen Labs Inc.</td><td>J0106LE</td><td>To decompress and drain ewe stomach</td></tr><tr><td>Hair clipper</td><td>Andis Company</td><td>65340</td><td>&amp;nbsp;# 40 Clipper Blade</td></tr><tr><td>Infant radiant warmer</td><td>GE healthcare</td><td>7810</td><td></td></tr><tr><td>Intravenous catheters</td><td>Becton Dickinson</td><td>381234</td><td></td></tr><tr><td>Iris surgical scissors</td><td>Patterson</td><td>510585</td><td></td></tr><tr><td>Kelly Foreceps</td><td>Patterson</td><td>510535</td><td></td></tr><tr><td>Mosquito Forceps</td><td>RICA Surgical Products INC</td><td>1-74</td><td></td></tr><tr><td>Near-infrared spectroscopy</td><td>Nonin Medical Inc.&amp;nbsp;</td><td>X-100M</td><td>Sensmart Equanox &amp;amp; PureSAT</td></tr><tr><td>RSO2 Sensor</td><td>Nonin Medical Inc.&amp;nbsp;</td><td>8004CB-NA</td><td>Neonatal&amp;nbsp;</td></tr><tr><td>Scalpel handle and blade</td><td>Everost</td><td>707203</td><td></td></tr><tr><td>Sutures -silk 2-0</td><td>Covidien</td><td>SS-695</td><td>Used for tying catheters to vessels</td></tr><tr><td>Sutures -vicryl&amp;nbsp; 2-0</td><td>Ethicon</td><td>J269H</td><td>Used for closing thoracotomy&amp;nbsp;</td></tr><tr><td>T-piece resuscitator</td><td>Neo-Tee</td><td>MCM1050812</td><td></td></tr><tr><td>Umbilical ties</td><td>Jorgensen Labs Inc.</td><td>J0025UA</td><td></td></tr><tr><td>Weitlander Retractor</td><td>Miltex</td><td>11-625</td><td></td></tr></tbody></table>

the perinatal asphyxiated lamb model: a model for newborn resuscitation

The Perinatal Asphyxiated Lamb Model: A Model for Newborn Resuscitation

Birth asphyxia accounts for nearly one million deaths worldwide each year, and is one of the primary causes of early neonatal morbidity and mortality. Many aspects of the current neonatal resuscitation guidelines remain controversial given the difficulties in conducting randomized clinical trials owing to the infrequent and often unpredictable need for extensive resuscitation. Most studies on neonatal resuscitation stem from manikin models that fail to truly reflect physiologic changes or piglet models that have cleared their lung fluid and that have completed the transition from fetal to neonatal circulation. The present protocol provides a detailed step-by-step description on how to create a perinatal asphyxiated fetal lamb model. The proposed model has a transitioning circulation and fluid-filled lungs, which mimics human newborns following delivery, and is, therefore, an excellent animal model to study newborn physiology. An important limitation to lamb experiments is the higher associated cost.

Following instrumentation of the fetal lambs, hemodynamic variables can be recorded (Figure 3 and Figure 4), then analyzed and interpreted (Figure 5). Frequent blood samples can be collected, and Figure 6 shows the pH and PaCO2 data from one of the experiments. Occasionally, catheters or flow probes may malfunction or get dislodged and data can, therefore, not...

Invasive instrumentation of the fetal lamb provides accurate physiologic measurements of the transitioning circulation in a model that closely mimics the newly born infant.

Birth asphyxia accounts for nearly one million deaths worldwide each year, and is one of the primary causes of early neonatal morbidity and mortality. ...

the-perinatal-asphyxiated-lamb-model-a-model-for-newborn-resuscitation

Research

JoVE Journal

Medicine

The postnatal rat model for hypoxic-ischemic brain injury is a well-established model of human neonatal hypoxic ischemic encephalopathy (HIE). In this article, we describe the model of HIE in post-natal rat pups.

The Hypoxic Ischemic Encephalopathy Model of Perinatal Ischemia

Large animal models have good translational values in the examination of physiology and pharmacology of neonatal asphyxia. Using newborn piglets, we develop an experimental protocol to simulate neonatal asphyxia which has advantages of studying the systemic and regional hemodynamics, oxygen transport with biochemical and pathologic pathways and correlations.

A Swine Model of Neonatal Asphyxia

Hypoxic-ischemic encephalopathy following perinatal asphyxia can be studied using animal models. We demonstrate the procedures necessary for establishing a piglet model of neonatal hypoxic-ischemic encephalopathy.

A Piglet Model of Neonatal Hypoxic-Ischemic Encephalopathy

Encephalopathy of prematurity encompasses the central nervous system abnormalities associated with injury from preterm birth. This report describes a clinically relevant rat model of in utero transient systemic hypoxia-ischemia and intra-amniotic lipopolysaccharide administration (LPS) that mimics chorioamnionitis, and the related impact of infectious stimuli and placental underperfusion on CNS development.

Modeling Encephalopathy of Prematurity Using Prenatal Hypoxia-ischemia with Intra-amniotic Lipopolysaccharide in Rats

This protocol is designed to describe a method to place and establish a supraglottic (alternative) airway during neonatal resuscitation. It can be used in situations where frontline providers cannot ventilate or endotracheally intubate neonates requiring resuscitation.

Laryngeal Mask Airway (LMA) Placement in a Neonatal Patient Simulator Using a Non-Inflatable Supraglottic Airway (SGA)

This piglet model involves surgical instrumentation, asphyxiation until the cardiac arrest, resuscitation, and post-resuscitation observation. The model allows for multiple sampling per animal, and by using continuous invasive arterial blood pressure, ECG, and non-invasive cardiac output monitoring, it provides knowledge about hemodynamics and cardiac pathophysiology in perinatal asphyxia and neonatal cardiopulmonary resuscitation.

A Piglet Perinatal Asphyxia Model to Study Cardiac Injury and Hemodynamics after Cardiac Arrest, Resuscitation, and the Return of Spontaneous Circulation

This protocol describes the development of a neonatal piglet model of acute lung injury that models early pathogenic events occurring in the preterm lung, including sub-adequate surfactant quantity, hyperoxia, high-pressure ventilation, and inflammation, to facilitate understanding of molecular triggers of bronchopulmonary dysplasia and enhance therapeutic translation.

Development of a Neonatal Piglet Acute Lung Injury Model Recreating the Early Environment of Preterm Infant Lungs

A powerful model for perioperative and critical care related acute kidney injury is presented.  Using whole body hypoperfusion induced by cardiac arrest it is possible to nearly replicate the histologic and functional changes of clinical AKI.

Normothermic Cardiac Arrest and Cardiopulmonary Resuscitation: A Mouse Model of Ischemia-Reperfusion Injury

This article describes a rat model of electrically-induced ventricular fibrillation and resuscitation by chest compression, ventilation, and delivery of electrical shocks that simulates an episode of sudden cardiac arrest and conventional cardiopulmonary resuscitation. The model enables gathering insights on the pathophysiology of cardiac arrest and exploration of new resuscitation strategies.

A Rat Model of Ventricular Fibrillation and Resuscitation by Conventional Closed-chest Technique

This protocol describes how to establish an enteroid culture system from neonatal mouse or premature human intestine as well as an efficient method to collect milk from mice.

Breast Milk Enhances Growth of Enteroids: An Ex Vivo Model of Cell Proliferation

Learn the perinatal asphyxiated lamb model for studying neonatal resuscitation and birth asphyxia using invasive instrumentation in fetal lambs. This method enables precise physiological measurements during fetal to neonatal transition.

The Perinatal Asphyxiated Lamb Model: A Model for Newborn Resuscitation

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