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An Ultrahigh-throughput Microfluidic Platform for Single-cell Genome Sequencing
This content is Free Access.
Chapters
- 00:05Title
- 00:48Encapsulation of Cells in Agarose Microgels
- 02:48Breaking the Agarose Microgels
- 03:54Generating Barcode Droplets by Digital PCR
- 04:53Tagmentation of Genomic DNA in Droplets
- 05:48Single-cell Barcoding by Microfluidic Double Merger
- 08:25Results: Ultrahigh Throughput Sequencing of Single-cell Genomes
- 09:24Conclusion
Single-cell sequencing reveals genotypic heterogeneity in biological systems, but current technologies lack the throughput necessary for the deep profiling of community composition and function. Here, we describe a microfluidic workflow for sequencing >50,000 single-cell genomes from diverse cell populations.
