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A Flow Cytometry-based Assay for Measuring Mitochondrial Membrane Potential in Cardiac Myocytes After Hypoxia/Reoxygenation
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A Flow Cytometry-based Assay for Measuring Mitochondrial Membrane Potential in Cardiac Myocytes After Hypoxia/Reoxygenation

A Flow Cytometry-based Assay for Measuring Mitochondrial Membrane Potential in Cardiac Myocytes After Hypoxia/Reoxygenation

DOI:
10.3791/57725-v

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07:14 min

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July 13, 2018

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, , ,
1State Key Laboratory of Cardiovascular Disease, Department of Cardiology, Fuwai Hospital, National Center for Cardiovascular Diseases,Chinese Academy of Medical Sciences and Peking Union Medical College, 2State Key Laboratory of Cardiovascular Disease, Fuwai Hospital, National Center for Cardiovascular Diseases,Chinese Academy of Medical Sciences and Peking Union Medical College

Chapters

  • 00:04Title
  • 00:42Establishment of Hypoxia/Reoxygenation Model
  • 01:57Preparation of HCMs for Flow Cytometry
  • 03:49Flow Cytometer Setup
  • 04:43Flow Cytometry Measurement and Data Analysis
  • 05:40Results: Validation of the Establishment of Hypoxia/Reoxygenation (H/R) Model by Flow Cytometry
  • 06:34Conclusion

Summary

Automatic Translation

Automatic Translation

Here, we present a protocol that uses JC-1 dye to assess the mitochondrial membrane potential of cells after being exposed to hypoxia/reoxygenation with or without a protective agent.

Tags

Flow CytometryMitochondrial Membrane PotentialCardiac MyocytesHypoxiaReoxygenationJC-1Cell CultureCentrifugationTrypsinizationPhosphate Buffer SalineDMEM

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