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Imaging Ca2+ Responses During Shigella Infection of Epithelial Cells
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Immunology and Infection
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JoVE Journal Immunology and Infection
Imaging Ca2+ Responses During Shigella Infection of Epithelial Cells

Imaging Ca2+ Responses During Shigella Infection of Epithelial Cells

DOI:
10.3791/57728-v

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08:56 min

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May 24, 2018

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, , ,
1Equipe Communication Intercellulaire et Infections Microbiennes, Centre de Recherche Interdisciplinaire en Biologie (CIRB),Collège de France, 2Institut National de la Santé et de la Recherche Médicale (Inserm), U1050, 3Centre Nationale de la Recherche Scientifique (CNRS), UMR7241, 4MEMOLIFE Laboratory of Excellence and Paris Sciences et Lettres, 5Inserm, UMRS1174,Université Paris Sud

Chapters

  • 00:04Title
  • 00:52Bacterial Preparation
  • 02:30Cell Preparation
  • 04:30Infection and Image Acquisition of Local Ca2 Responses
  • 06:50Results: Calcium Imaging During Shigella Infection
  • 08:21Conclusion

Summary

Automatic Translation

Automatic Translation

Here, we present protocols to visualize calcium (Ca2+) responses elicited by HeLa cells infected by Shigella. By optimizing the parameters of bacterial infection and imaging with Ca2+ fluorescent probes, atypical global and local Ca2+ signals induced by bacteria over a large range of infection kinetics are characterized.

Tags

Keywords: Ca2+ ImagingShigella InfectionEpithelial CellsFluorescent MicroscopyCalcium SignalingPathogen-induced SignalingCell DeathCytoskeletal ReorganizationChemical ProbesAfaE AdhesinHeLa CellsTC7 CellsCell CultureTrypsinizationCell CountingCell Polarization

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