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JoVE Journal
Immunology and Infection

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Imaging Ca2+ Responses During Shigella Infection of Epithelial Cells
 

Imaging Ca2+ Responses During Shigella Infection of Epithelial Cells

Article doi: 10.3791/57728
May 24th, 2018

Summary May 24th, 2018

Here, we present protocols to visualize calcium (Ca2+) responses elicited by HeLa cells infected by Shigella. By optimizing the parameters of bacterial infection and imaging with Ca2+ fluorescent probes, atypical global and local Ca2+ signals induced by bacteria over a large range of infection kinetics are characterized.

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