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Journal / Immunology and Infection / Encellede kvantitering af mRNA og overflade…
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Immunology and Infection

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JoVE Journal Immunology and Infection
Encellede kvantitering af mRNA og overflade Protein udtryk i Simian immundefektvirus-inficerede CD4+ T celler isoleret fra Rhesus makakaber
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Encellede kvantitering af mRNA og overflade Protein udtryk i Simian immundefektvirus-inficerede CD4+ T celler isoleret fra Rhesus makakaber

Article DOI: 10.3791/57776-v • 13:13 min • September 25th, 2018
September 25th, 2018 •
1, 1, 1, 2, 1
1US Military HIV Research Program, Henry M. Jackson Foundation, Walter Reed Army Institute of Research, 2Vaccine Research Center, NIAID, NIH

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Beskrevet er en metode til at kvantificere udtryk for 96 gener og 18 overflade proteiner af enkelt celler ex vivo, giver mulighed for identifikation af varierende udtrykte gener og proteiner i virus-inficerede celler i forhold til ikke-inficerede celler. Vi anvender tilgang til undersøgelse SIV-inficerede CD4+ T celler isoleret fra rhesus makakaber.

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Immunologi og infektion sag 139 enkelt celle SIV mRNA multipleksede qPCR indekseret flowcytometri rhesus makakaber
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