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Tracing Gene Expression Through Detection of β-galactosidase Activity in Whole Mouse Embryos
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JoVE Journal Developmental Biology
Tracing Gene Expression Through Detection of β-galactosidase Activity in Whole Mouse Embryos

Tracing Gene Expression Through Detection of β-galactosidase Activity in Whole Mouse Embryos

DOI:
10.3791/57785-v

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08:42 min

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June 26, 2018

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, , , , , ,
1Faculty of Biomedical and Health Sciences,Universidad Europea de Madrid, 2Centro Nacional de Investigaciones Cardiovasculares Carlos III (CNIC), 3School of Doctoral Studies and Research,Universidad Europea de Madrid

Chapters

  • 00:04Title
  • 00:49β-galactosidase Histochemistry of Whole Mount Mouse Embryos
  • 02:23Paraffin Embedding of X-gal Stained Embryos
  • 04:37Paraffin Sectioning
  • 05:44Deparaffinization and Counterstaining
  • 06:56Results: X-gal Staining of E9.5 WT and Mt4-mmpLacZ/ Whole-mount Embryos and Tissue Sections
  • 07:52Conclusion

Summary

Automatic Translation

Automatic Translation

Here we describe the standard protocol for the detection of β-galactosidase activity in early whole mouse embryos and the method for paraffin sectioning and counterstaining. This is an easy and quick procedure to monitor gene expression during development that can also be applied to tissue sections, organs or cultured cells.

Tags

Keywords: β-galactosidaseGene ExpressionWhole MountX-gal StainingMouse EmbryosDevelopmental BiologyReporter GeneLineage TracingHistochemical DetectionParaffin Sectioning

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