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JoVE Journal
Bioengineering

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In Situ Microscopy for Real-time Determination of Single-cell Morphology in Bioprocesses
 

In Situ Microscopy for Real-time Determination of Single-cell Morphology in Bioprocesses

Article DOI: 10.3791/57823-v
December 5th, 2019

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Summary December 5th, 2019

A photo-optical in situ microscopy device was developed to monitor the size of single cells directly in the cell suspension. The real-time measurement is conducted by coupling the photo-optical sterilizable probe to an automated image analysis. Morphological changes appear with dependence on the growth state and cultivation conditions.

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