In Situ Microscopy for Real-time Determination of Single-cell Morphology in Bioprocesses

This method can help answer key questions of population heterogeneity through the morphological assessment of the cells. In situ microscopy allows study cultivation conditions, process yields, and product quality. The main advantage of this technique is an in situ measurement, which provides real-time data without sampling or bringing risk of contamination.

In situ microscopy coupled to automated image recognition can provide additional information about cellular structures, shape, and cell agglomeration beyond size. It's also possible to measure cell concentrations. The method has proven to be a reliable tool.