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JoVE Journal
Bioengineering

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A Rapid Image-based Bacterial Virulence Assay Using Amoeba
 

A Rapid Image-based Bacterial Virulence Assay Using Amoeba

Article doi: 10.3791/57844
June 27th, 2018

Summary June 27th, 2018

Here, we present a protocol to measure the virulence of planktonic or surface-attached bacteria using D. discoideum (amoeba) as a host. Virulence is measured over a period of 1 h and host killing is quantified using fluorescence microscopy and image analysis. We demonstrate this protocol using the bacterium P. aeruginosa.

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