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Analysis of Beta-cell Function Using Single-cell Resolution Calcium Imaging in Zebrafish Islets

Article DOI: 10.3791/57851-v • 08:50 min • July 3rd, 2018
July 3rd, 2018 •
1,2, 1, 1,2
1Center for Molecular and Cellular Bioengineering, TU Dresden, 2Paul Langerhans Institute Dresden of the Helmholtz Center Munich at the University Hospital Carl Gustav Carus of TU Dresden

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Summary
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Beta-cell functionality is important for blood-glucose homeostasis, which is evaluated at single-cell resolution using a genetically encoded reporter for calcium influx.

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Beta-cell Function Single-cell Resolution Calcium Imaging Zebrafish Islets Function Heterogeneity Spatial Resolution Temporal Resolution Live Imaging Calcium Oscillations Fish Euthanization Petrie Dish HBSS Solution Sharp Forceps Red Fluorescence MKO2 Expression Primary Islet Surrounding Tissue Removal Glass-bottomed Dish HBSS Washing Fibrinogen Working Solution Thrombin Solution
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