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JoVE Journal
Immunology and Infection

This content is Open Access.

Long-term In Vivo Tracking of Inflammatory Cell Dynamics Within Drosophila Pupae
 

Long-term In Vivo Tracking of Inflammatory Cell Dynamics Within Drosophila Pupae

Article DOI: 10.3791/57871 09:26 min June 14th, 2018
June 14th, 2018

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Summary

Here we present a protocol for live-imaging wound repair and the associated inflammatory response at high spatio-temporal resolution in vivo. This method utilizes the pupal stage of Drosophila development to enable long-term imaging and tracking of specific cell populations over time and is compatible with efficient RNAi-mediated gene inactivation.

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Long-term In Vivo Tracking Inflammatory Cell Dynamics Drosophila Pupae Imaging Potential Genetic Tractability Wound Healing Innate Immunity Live Imaging Traditional Models Tissue Area Experimental Manipulation Hemocytes RNAi Mediated Gene Inactivation Pupae Collection Vials White Pre-pupae Forceps Paint Brush Fly Vial Double Sided Sticky Tape Glass Slide Bright Field Dissection Microscope Incision
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