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Long-term In Vivo Tracking of Inflammatory Cell Dynamics Within Drosophila Pupae
This content is Free Access.
1School of Biochemistry, Biomedical Sciences,University of Bristol, 2School of Cellular and Molecular Medicine, Biomedical Sciences,University of Bristol, 3MRC Centre for Inflammation Research,University of Edinburgh, Queens Medical Research Institute, 4School of Physiology, Pharmacology, and Neuroscience, Biomedical Sciences,University of Bristol
Chapters
- 00:04Title
- 00:51Preparing the Pupae
- 03:12Laser-induced Wounding of Drosophila Pupal Wings
- 04:41In Vivo Time-lapse Confocal Imaging
- 06:52Results: Sterile Wounding Activates a Robust Inflammatory Response in the Drosophila Pupal Wing
- 08:28Conclusion
Here we present a protocol for live-imaging wound repair and the associated inflammatory response at high spatio-temporal resolution in vivo. This method utilizes the pupal stage of Drosophila development to enable long-term imaging and tracking of specific cell populations over time and is compatible with efficient RNAi-mediated gene inactivation.
