Laser-induced Wounding
Laser-induced Wounding - Related Videos
03:21
Epidermal Laser Wounding: Inducing Localized Damage to Examine Repair Processes in C. elegans
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2023
This video introduces a method of laser wounding, which is used to cause very precise injuries in the worm epidermis. The sample protocol is from a study of wound response and repair.
08:07
Laser-induced Forward Transfer of Ag Nanopaste
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Cited by 13 •
2016
We demonstrate the use of the Laser-induced forward transfer technique (LIFT) for the printing of high-viscosity Ag paste. This technique offers a simple, low temperature, robust process for non-lithographically printing microscale 2D and 3D structures.
08:35
Demonstration of the Rat Ischemic Skin Wound Model
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Cited by 32 •
2015
The rat, due to its size, availability, and rather docile behavior, has been utilized as a research model for many years. The goal of this protocol is to utilize the rat as an ischemic skin wound healing model to provide valuable insight into the pathophysiology of chronic wounds.
07:08
A Mouse Model for Laser-induced Choroidal Neovascularization
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Cited by 87 •
2015
Here, we present the mouse laser-induced choroidal neovascularization (CNV) protocol, an experimental model that re-creates the vascular hallmarks of neovascular age-related macular degeneration (AMD). Once mastered, it can reliably and effectively induce CNV as a model system to test various experimental measures.
02:48
Colonoscopic Guided Murine Wound Model: A Method to Isolate Biopsy Induced Wound Bed from Colon to Understand the Healing Process
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2025
In this video, we describe a procedure to isolate the colonoscopic-induced wound bed at an appropriate time point for detailed molecular analysis. This analysis helps to understand the wound healing process in inflammatory conditions of the GI tract.
FAQs
The laser’s focused energy determines where tissue damage occurs: energy deposition can produce localized ablation or thermal injury in the selected area while limiting damage around it. This spatial control lets investigators associate observed barrier disruption, cell death, or immune-cell recruitment with a defined lesion rather than with broadly distributed tissue injury.
These lesions connect three measurable events: disruption of a barrier, death of affected cells, and recruitment of immune cells. Examining them together helps researchers distinguish the immediate consequences of tissue damage from the subsequent inflammatory response. That distinction is important for studying how inflammation begins and how innate immune defenses respond to injury.
Temporal precision allows a defined lesion to be followed as the response develops, rather than assessed only as a final endpoint. Researchers can therefore examine the relationship between initial damage, emerging inflammation, immune-cell recruitment, and later repair. This time-resolved approach supports quantitative studies of how wound responses change after the injury is created.
Researchers first use focused laser energy to create a precise lesion in a cell, tissue, or living model organism. They then monitor the resulting barrier disruption, cell death, immune-cell recruitment, or repair response. In infection studies, the same workflow can be used to examine pathogen interactions with the injured site and associated antimicrobial responses.
In immunology, a controlled lesion provides a defined starting point for examining how inflammation begins after tissue damage. Monitoring immune-cell recruitment and innate defense responses reveals how the organism reacts to a localized injury. The approach also supports quantitative analysis of wound repair, linking early inflammatory events with subsequent tissue responses.
The method creates an injured site whose interaction with pathogens can be monitored in a controlled spatial and temporal context. Researchers can investigate whether and how pathogens engage damaged tissue while examining accompanying antimicrobial responses. This makes the approach useful for connecting tissue injury, host defense, and infection-related outcomes within the same experimental system.