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JoVE Journal
Biochemistry

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In Situ Measurement and Correlation of Cell Density and Light Emission of Bioluminescent Bacteria
 

In Situ Measurement and Correlation of Cell Density and Light Emission of Bioluminescent Bacteria

Article doi: 10.3791/57881
June 28th, 2018 Usage Statistics

Summary June 28th, 2018

Bioluminescent bacteria regulate light production through a variety of mechanisms, such as quorum sensing. This novel method allows the in situ investigation of bioluminescence and the correlation of light emission to cell density. An artificial bioluminescent Escherichia coli system allows the characterization of lux operons, Lux proteins, and their interplay.

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