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Biochemistry

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JoVE Journal Biochemistry
In Situ Measurement and Correlation of Cell Density and Light Emission of Bioluminescent Bacteria
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In Situ Measurement and Correlation of Cell Density and Light Emission of Bioluminescent Bacteria

Article DOI: 10.3791/57881-v • 05:52 min • June 28th, 2018
June 28th, 2018 •
1, 1, 1
1Institute of Biochemistry, Graz University of Technology

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Summary
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Bioluminescent bacteria regulate light production through a variety of mechanisms, such as quorum sensing. This novel method allows the in situ investigation of bioluminescence and the correlation of light emission to cell density. An artificial bioluminescent Escherichia coli system allows the characterization of lux operons, Lux proteins, and their interplay.

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In Situ Measurement Correlation Cell Density Light Emission Bioluminescent Bacteria Optimal Growth Conditions Maximal Light Intensity Regulatory Mechanism Easy Setup Adjustment Possibilities Lux Operon Characteristics Bacterial Systems Environmental Systems Reporting Systems Sensors Overnight Culture LB Medium Kanamycin E.coli BL21 Cells Glycerol Stock Transformation Plate Incubation Temperature Protein Expression IPTG Artificial Sea Water Medium Agar Plates
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