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Isolation and Analysis of Microbial Communities in Soil, Rhizosphere, and Roots in Perennial Grass Experiments
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Isolation and Analysis of Microbial Communities in Soil, Rhizosphere, and Roots in Perennial Grass Experiments

Isolation and Analysis of Microbial Communities in Soil, Rhizosphere, and Roots in Perennial Grass Experiments

DOI:
10.3791/57932-v

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10:31 min

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July 24, 2018

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, , , ,
1Department of Agronomy and Horticulture and Center for Plant Science Innovation,University of Nebraska-Lincoln, 2USDA-ARS, Wheat, Sorghum and Forage Research Unit,University of Nebraska-Lincoln East Campus

Chapters

  • 00:04Title
  • 00:36Plant Excavation, Soil Removal, and Bulk Soil Collection
  • 01:46Root and Rhizosphere Collection
  • 02:44Root Surface Sterilization
  • 03:54Processing Rhizosphere Samples
  • 05:02DNA Extraction and Soil Analysis Processing
  • 06:33DNA Extraction in 96-well Format
  • 07:45Rhizosphere Sample Loading
  • 08:47Results: Visualization and Statistical Methods Highlight Differences in Microbial Community Composition and Diversity Between Sample Types and Grass Species
  • 10:03Conclusion

Summary

Automatic Translation

Automatic Translation

Excavation of plant roots from the field as well as processing of samples into endosphere, rhizosphere, and soil are described in detail, including DNA extraction and data analysis methods. This paper is designed to enable other laboratories to use these techniques for the study of soil, endosphere, and rhizosphere microbiomes.

Tags

Keywords: Microbial CommunitiesSoilRhizosphereRoot EndospherePerennial GrassField ExperimentsSample CollectionSoil SamplingRoot SamplingRhizosphere ExtractionRoot SterilizationMicrobiome Analysis

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