Digital PCR for Quantifying Circulating MicroRNAs in Acute Myocardial Infarction and Cardiovascular Disease

Louise Benning; Samuel Robinson; Marie Follo; Lukas Andreas Heger; Daniela Stallmann; Daniel Duerschmied; Christoph Bode; Ingo Ahrens; Marcus Hortmann

doi:10.3791/57950

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Medicine

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PCR digitale per quantificare circolanti microRNA nell'infarto miocardico acuto e la malattia cardiovascolare

Digital PCR for Quantifying Circulating MicroRNAs in Acute Myocardial Infarction and Cardiovascular Disease

JoVE Journal
Medicine

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JoVE Journal Medicine

Digital PCR for Quantifying Circulating MicroRNAs in Acute Myocardial Infarction and Cardiovascular Disease

PCR digitale per quantificare circolanti microRNA nell'infarto miocardico acuto e la malattia cardiovascolare

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8,036 Views

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04:41 min

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July 03, 2018

DOI:

10.3791/57950-v

DOI:

10.3791/57950-v

•

04:41 min

July 03, 2018

•

4 Views

Louise Benning¹, Samuel Robinson^1,2, Marie Follo³, Lukas Andreas Heger¹, Daniela Stallmann¹, Daniel Duerschmied¹, Christoph Bode¹, Ingo Ahrens^1,4, Marcus Hortmann¹

¹Department of Cardiology and Angiology I, Heart Center Freiburg University, Faculty of Medicine,University of Freiburg, ²Department of Medicine,Monash University, ³Department of Medicine I, Lighthouse Core Facility, Medical Center,University of Freiburg, ⁴Department of Cardiology, Augustinerinnen Hospital, Academic Teaching Hospital,University of Cologne

Transcript

Summary

MicroRNA circolanti hanno indicato la promessa come biomarcatori per malattie cardiovascolari e infarti miocardici acuti. In questo studio, descriviamo un protocollo per l'estrazione di miRNA, trascrizione d'inversione e digitali PCR per la quantificazione assoluta dei miRNA nel siero dei pazienti con la malattia cardiovascolare.