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A Temperature Gradient Assay to Determine Thermal Preferences of Drosophila Larvae
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JoVE Journal Neuroscience
A Temperature Gradient Assay to Determine Thermal Preferences of Drosophila Larvae

A Temperature Gradient Assay to Determine Thermal Preferences of Drosophila Larvae

DOI:
10.3791/57963-v

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08:59 min

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June 25, 2018

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, ,
1Neuroscience Research Institute and Department of Molecular, Cellular and Developmental Biology,University of California, Santa Barbara, 2Division of Cell Signaling, National Institute for Physiological Sciences,National Institutes of Natural Sciences, 3Thermal Biology Group, Exploratory Research Center on Life and Living Systems,National Institutes of Natural Sciences

Chapters

  • 00:05Title
  • 00:43Larval Synchronization
  • 02:03Single-directional Temperature Gradient Setup
  • 03:30Larval Collection and Washing
  • 05:08Assay and Calculation
  • 06:25Results: Larvae Distribution from 18 °C – 28 °C
  • 08:13Conclusion

Summary

Automatic Translation

Automatic Translation

Here, we present a protocol to determine the preferred environmental temperature of Drosophila larvae using a continuous thermal gradient.

Tags

Keywords: Temperature GradientThermal PreferenceDrosophila LarvaeSomatosensationTemperature AssayC. ElegansYeast PasteEgg CollectionAgarose PlateAluminum BlocksWater Bath

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