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Spectral Reflectometric Microscopy on Myelinated Axons In Situ
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Neuroscience
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JoVE Journal Neuroscience
Spectral Reflectometric Microscopy on Myelinated Axons In Situ

Spectral Reflectometric Microscopy on Myelinated Axons In Situ

DOI:
10.3791/57965-v

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09:13 min

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July 02, 2018

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,
1Department of Biomedical Engineering,Sungkyunkwan University, 2Center for Neuroscience Imaging Research,Institute for Basic Science (IBS)

Chapters

  • 00:04Title
  • 00:41Mounting Brain Slices
  • 01:41Calibration
  • 04:06SpeRe Image Acquisition
  • 05:03Image Processing and Analysis
  • 06:10Baseline Correction and SpeRe Signal Analysis
  • 07:06Results: Spectral Reflectometric Microscopy on Myelinated Axons
  • 08:28Conclusion

Summary

Automatic Translation

Automatic Translation

Here, we present a step-by-step protocol for imaging myelinated axons in a fixed brain slice using a label-free nanoscale imaging technique based on spectral reflectometry.

Tags

Keywords: Spectral Reflectometric MicroscopyMyelinated AxonsIn SituMyelin PlasticityDemyelinationGlass SlideCover GlassSpacerPBSNail PolishWhite Light LaserPhotomultiplier TubeLambda ScanWater-immersion ObjectiveAOBS ConfigurationReference Mirror

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