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Journal / Genetics / 酿酒酵母4-硫尿嘧啶代谢标记及新合成 mRNA 作为 RNA 聚合酶 II.…
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Genetics

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JoVE Journal Genetics
酿酒酵母4-硫尿嘧啶代谢标记及新合成 mRNA 作为 RNA 聚合酶 II. 活性的一种代理
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酿酒酵母4-硫尿嘧啶代谢标记及新合成 mRNA 作为 RNA 聚合酶 II. 活性的一种代理

Article DOI: 10.3791/57982-v • 09:21 min • October 22nd, 2018
October 22nd, 2018 •
1,2,3,4, 1,2,3,4
1Institut de Génétique et de Biologie Moléculaire et Cellulaire, Illkirch, France, 2Centre National de la Recherche Scientifique, Illkirch, France, 3Institut National de la Santé et de la Recherche Médicale, Illkirch, France, 4Université de Strasbourg

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这里描述的协议是基于全基因组的定量的新合成的 mRNA 纯化的酵母细胞标记为 4-硫尿嘧啶。这种方法允许测量 mrna 合成与 mrna 衰变分离, 从而提供了 RNA 聚合酶 II 转录的精确测量。

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行为 问题 140 S. 酵母 rna 转录 新合成的 rna 4-硫尿嘧啶 RNA 聚合酶 II 佐贺复合体
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