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JoVE Journal Neuroscience
Simple Surgical Induction of Conductive Hearing Loss with Verification Using Otoscope Visualization and Behavioral Clap Startle Response in Rat
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Simple Surgical Induction of Conductive Hearing Loss with Verification Using Otoscope Visualization and Behavioral Clap Startle Response in Rat

Article DOI: 10.3791/57993-v • 06:27 min • October 26th, 2019
October 26th, 2019 •
*1, *2,3,4, 5, 1, 5, 2,3,6, 4
1Instituto de Neurobiología, Universidad Nacional Autónoma de México, México, 2State Key Laboratory of Marine Pollution (SKLMP), City University of Hong Kong, Hong Kong, 3Department of Biomedical Sciences, College of Veterinary Medicine and Life Sciences, City University of Hong Kong, Hong Kong, 4Department of Physics, City University of Hong Kong, Hong Kong, 5Department of Clinical Oncology, Queen Elizabeth Hospital, Hong Kong, 6Department of Materials Science and Engineering, City University of Hong Kong, Hong Kong
* These authors contributed equally

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Summary
Automatic Translation

Here, we present a protocol to establish a replicable conductive hearing loss induction via surgical tympanic membrane puncture and verification by otoscope visualization and behavioral assessment by clap startle.

Tags

Surgical Induction Conductive Hearing Loss Tympanic Membrane Puncture Otoscope Visualization Behavioral Clap Startle Response Rat Human Hearing Conductive Disorders Malleus Removal Non-invasive Procedures Validation Animal Research Ethics Committee City University Of Hong Kong University Of Hong Kong Department Of Health Of The Hong Kong Special Administrative Region Anesthesia Ketamine Xylazine IP Injection Pain Sensation
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