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RNA de la guía CRISPR clonación de mamíferos sistemas

CRISPR Guide RNA Cloning for Mammalian Systems

JoVE Journal
Genetics

This content is Free Access.

JoVE Journal Genetics

CRISPR Guide RNA Cloning for Mammalian Systems

RNA de la guía CRISPR clonación de mamíferos sistemas

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70,444 Views

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06:48 min

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October 02, 2018

DOI:

10.3791/57998-v

DOI:

10.3791/57998-v

•

06:48 min

October 02, 2018

•

70211 Views

Sathiji Nageshwaran*^1,2, Alejandro Chavez*^1,2,3, Nan Cher Yeo^1,2, Xiaoge Guo^1,2, Alissa Lance-Byrne¹, Angela Tung¹, James J. Collins^1,4,5,6,7, George M. Church^1,2

¹Wyss Institute for Biologically Inspired Engineering,Harvard University ²Department of Genetics,Harvard Medical School ³Department of Pathology,Massachusetts General Hospital ⁴Institute for Medical Engineering & Science,Massachusetts Institute of Technology ⁵Synthetic Biology Center,Massachusetts Institute of Technology ⁶Department of Biological Engineering,Massachusetts Institute of Technology ⁷Broad Institute

Summary

Aquí, una simple, eficaz y rentable método de clonación sgRNA se contornea.

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