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JoVE Journal
Developmental Biology

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Single-cell Transcriptomic Analyses of Mouse Pancreatic Endocrine Cells
 

Single-cell Transcriptomic Analyses of Mouse Pancreatic Endocrine Cells

Article doi: 10.3791/58000
September 30th, 2018

Summary September 30th, 2018

We describe a method for the isolation of endocrine cells from embryonic, neonatal and postnatal pancreases followed by single-cell RNA sequencing. This method allows analyses of pancreatic endocrine lineage development, cell heterogeneity and transcriptomic dynamics.

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