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荧光标记蛋白 ImageJ 的无偏分析在
果蝇
变性定量细胞生物学中的应用
JoVE Journal
Neuroscience
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JoVE Journal
Neuroscience
Quantitative Cell Biology of Neurodegeneration in
Drosophila
Through Unbiased Analysis of Fluorescently Tagged Proteins Using ImageJ
Please note that all translations are automatically generated.
Click here for the English version.
荧光标记蛋白 ImageJ 的无偏分析在
果蝇
变性定量细胞生物学中的应用
DOI:
10.3791/58041-v
•
08:44 min
•
August 03, 2018
•
Jennifer M. Brazill
,
Yi Zhu
,
Chong Li
,
R. Grace Zhai
2
1
Department of Molecular and Cellular Pharmacology
,
University of Miami Miller School of Medicine
,
2
School of Pharmacy, Key Laboratory of Molecular Pharmacology and Drug Evaluation (Yantai University), Ministry of Education, Collaborative Innovation Center of Advanced Drug Delivery System and Biotech Drugs in Universities of Shandong
,
Yantai University
Chapters
00:04
Title
00:50
Brain Dissection and Immunofluorescence Staining
02:23
Image Acquisition and Region of Interest (ROI) Selection
04:19
Preprocessing and Segmentation with H-Maxima Watershedding and Quantity, Area, and Intensity Quantification
06:30
Results: Representative Quantitative Analysis of Cell Biology of Neurodegeneration
08:20
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
我们开发了一个简单且适应性强的工作流, 从荧光成像的细胞生物学研究中提取定量数据自噬性
果蝇
模型中中枢神经系统蛋白质聚集和通量变性.
Tags
Quantitative Cell Biology
Neurodegeneration
Drosophila
Fluorescently Tagged Proteins
ImageJ
Image Analysis Workflow
Proteinaceous Puncta
Membrane-bound Compartments
Neurodegenerative Diseases
Lamina Dissection
Retina Removal
Brain Dissection
Immunostaining
Antibody Incubation
Mounting
Microscopy
Fluorescence Imaging
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