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荧光标记蛋白 ImageJ 的无偏分析在果蝇变性定量细胞生物学中的应用
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
Quantitative Cell Biology of Neurodegeneration in Drosophila Through Unbiased Analysis of Fluorescently Tagged Proteins Using ImageJ
DOI:

08:44 min

August 03, 2018

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Chapters

  • 00:04Title
  • 00:50Brain Dissection and Immunofluorescence Staining
  • 02:23Image Acquisition and Region of Interest (ROI) Selection
  • 04:19Preprocessing and Segmentation with H-Maxima Watershedding and Quantity, Area, and Intensity Quantification
  • 06:30Results: Representative Quantitative Analysis of Cell Biology of Neurodegeneration
  • 08:20Conclusion

Summary

Automatic Translation

我们开发了一个简单且适应性强的工作流, 从荧光成像的细胞生物学研究中提取定量数据自噬性果蝇模型中中枢神经系统蛋白质聚集和通量变性.

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