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Choice and No-Choice Bioassays to Study the Pupation Preference and Emergence Success of Ectropis grisescens
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Choice and No-Choice Bioassays to Study the Pupation Preference and Emergence Success of Ectropis grisescens

Article DOI: 10.3791/58126-v • 05:19 min • October 30th, 2018
October 30th, 2018 •
1, 1, 1,2, 3, 4, 5, 1, 1, 1, 1
1Guangdong Key Laboratory for Innovation Development and Utilization of Forest Plant Germplasm, College of Forestry and Landscape Architecture, South China Agricultural University, 2College of Agriculture, South China Agricultural University, 3Tea Research Institute, Chinese Academy of Agricultural Sciences, 4College of Horticulture, South China Agricultural University, 5College of Coast and Environment, Louisiana State University

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Summary
Automatic Translation

Here, we present a protocol to investigate the pupation preference of mature larvae of Ectropis grisescens in response to soil factors (e.g., substrate type and moisture content) using choice bioassays. We also present a protocol of no-choice bioassays to determine the factors that affect the pupation behaviors and survivorship of E. grisescens.

Tags

Choice Bioassay No-choice Bioassay Ectropis Grisescens Pupation Preference Emergence Success Pupation Ecology Environmental Factors Tea Leaf Caterpillars Measuring Pupation Preference Emergence Success Fresh Shoots Triangular Flask Tap Water Plastic Basin Larvae Release Laboratory Conditions Last-instar Larvae Sterilize Soil And Sand Substrate Samples Wooden Pestle And Mortar Sieve Distilled Water PVC Sheets
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