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JoVE Journal
Neuroscience

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Preparation of a High-quality Primary Cell Culture from Fish Pituitaries
 

Preparation of a High-quality Primary Cell Culture from Fish Pituitaries

Article doi: 10.3791/58159
August 28th, 2018

Summary August 28th, 2018

Here we describe a protocol to prepare and maintain primary pituitary cell cultures from medaka (Oryzias latipes). The optimized conditions in this protocol take important parameters such as temperature, osmolality, and pH into consideration by mimicking the physiological conditions of the fish, thereby enabling physiologically more meaningful results.

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