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Preparation of a High-quality Primary Cell Culture from Fish Pituitaries
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Neuroscience
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JoVE Journal Neuroscience
Preparation of a High-quality Primary Cell Culture from Fish Pituitaries

Preparation of a High-quality Primary Cell Culture from Fish Pituitaries

DOI:
10.3791/58159-v

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07:36 min

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August 28, 2018

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, , , , ,
1Department of Basic Sciences and Aquatic Medicine, Faculty of Veterinary Medicine,Norwegian University of Life Sciences, 2Department of Oral Biology, Faculty of Dentistry,University of Oslo

Chapters

  • 00:04Title
  • 00:34Pituitary Dissection and Cell Dissociation
  • 05:58Results: Dissociated Medaka Pituitary Cell Viability in Primary Cell Culture
  • 07:07Conclusion

Summary

Automatic Translation

Automatic Translation

Here we describe a protocol to prepare and maintain primary pituitary cell cultures from medaka (Oryzias latipes). The optimized conditions in this protocol take important parameters such as temperature, osmolality, and pH into consideration by mimicking the physiological conditions of the fish, thereby enabling physiologically more meaningful results.

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Keywords: Primary Cell CultureFish PituitaryMedakaEndocrinologyTrypsinTrypsin InhibitorL-15 MediumPoly-D-lysineCell Culture Dish

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