Derivation and Differentiation of Canine Ovarian Mesenchymal Stem Cells

Amanda B.T. Hill; Jonathan E.B.T. Hill; Fabiana F. Bressan; Maria Ang&eacute;lica Miglino; Joaquim M. Garcia

doi:10.3791/58163

Journal

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Developmental Biology

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Derivation and Differentiation of Canine Ovarian Mesenchymal Stem Cells

JoVE Journal
Developmental Biology
Author Produced

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JoVE Journal Developmental Biology

Derivation and Differentiation of Canine Ovarian Mesenchymal Stem Cells

5,608 Views

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11:41 min

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December 16, 2018

DOI:

10.3791/58163-v

DOI:

10.3791/58163-v

•

11:41 min

December 16, 2018

•

4 Views

Amanda B.T. Hill^1,2, Jonathan E.B.T. Hill¹, Fabiana F. Bressan³, Maria Angélica Miglino⁴, Joaquim M. Garcia¹

¹The School of Agricultural and Veterinary Studies (FCAV), Jaboticabal,São Paulo State University (UNESP), ²CRRF,Université de Montréal, ³FZEA,University of São Paulo, ⁴FMVZ,University of São Paulo

Transcript

The overall goal of this procedure is to isolate, expand and differentiate mesenchymal stems sells derived from canine ovarian tissue. This experiment was performed with the ovaries of four mongrel female dogs donated after elective surgery at a canine sterilization program. This experiment was approved by the Ethics Committee on the use of animals of Sao Paulo State University, FVAC.

Representative results.Discussion.Conclusion.