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Journal / Immunology and Infection / Kvantitativ PCR af T7 Bacteriophage fra Biopanning
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Immunology and Infection

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Kvantitativ PCR af T7 Bacteriophage fra Biopanning

Article DOI: 10.3791/58165-v • 05:42 min • September 27th, 2018
September 27th, 2018 •
1, 1, 1, 1, 1
1Division of Molecular Pharmaceutics and Drug Delivery, College of Pharmacy, University of Texas at Austin

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En reproducerbar, præcis og tid effektiv kvantitativ PCR (qPCR) metode til at optælle T7 bacteriophage er beskrevet her. Protokollen beskriver klart phage genomisk DNA forberedelse, PCR reaktion forberedelse, qPCR cykling betingelser og qPCR dataanalyse.

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Immunologi og infektion sag 139 T7 phage phage genomisk DNA qPCR plaque assay påvisningsgrænse kvantificering vifte
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