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Journal / Immunology and Infection / T7 bakteriyofaj Biopanning üzerinden kantitatif…
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T7 bakteriyofaj Biopanning üzerinden kantitatif PCR

Article DOI: 10.3791/58165-v • 05:42 min • September 27th, 2018
September 27th, 2018 •
1, 1, 1, 1, 1
1Division of Molecular Pharmaceutics and Drug Delivery, College of Pharmacy, University of Texas at Austin

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T7 bakteriyofaj numaralandırmak için bir tekrarlanabilir, doğru ve zamanı verimli kantitatif PCR (qPCR) yöntemi burada açıklanmıştır. Protokol açıkça fajının genomik DNA hazırlık, PCR reaksiyon hazırlama, qPCR Bisiklet koşullarını ve qPCR veri analizi açıklar.

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İmmünoloji ve enfeksiyon sayı: 139 T7 faj fajının genomik DNA qPCR plak tahlil algılama miktar aralığı sınırı
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