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JoVE Journal
Immunology and Infection

This content is Open Access.

Generating Recombinant Avian Herpesvirus Vectors with CRISPR/Cas9 Gene Editing
 

Generating Recombinant Avian Herpesvirus Vectors with CRISPR/Cas9 Gene Editing

Article doi: 10.3791/58193
January 7th, 2019 Usage Statistics

Summary January 7th, 2019

Herpesvirus of turkeys (HVT) is widely used as a vector platform for the generation of recombinant vaccines against a number of avian diseases. This article describes a simple and rapid approach for the generation of recombinant HVT-vectored vaccines using an integrated NHEJ-CRISPR/Cas9 and Cre-Lox system.

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