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A High-throughput Platform for the Screening of Salmonella spp./Shigella spp.
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Immunology and Infection
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JoVE Journal Immunology and Infection
A High-throughput Platform for the Screening of Salmonella spp./Shigella spp.

A High-throughput Platform for the Screening of Salmonella spp./Shigella spp.

DOI:
10.3791/58200-v

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06:55 min

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November 07, 2018

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, , , ,
1State Key Laboratory of Diarrhea Disease Detection,Zhuhai International Travel Healthcare Center

Chapters

  • 00:04Title
  • 01:06Real-time PCR
  • 02:07Salmonella spp. PCR Positive Sample
  • 03:41Shigella spp. PCR Positive Sample
  • 04:38Results: Stool Samples Screened for Salmonella and Shigella spp.
  • 05:59Conclusion

Summary

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Automatic Translation

Salmonella spp./Shigella spp. are common pathogens attributed to diarrhea. Here, we describe a high-throughput platform for the screening of Salmonella spp./Shigella spp. using real-time PCR combined with guided culture.

Tags

Keywords: SalmonellaShigellaHigh-throughput ScreeningNAATReal-time PCRCulture IdentificationPathogensVibrioStaphylococcusE. ColiPre-enrichmentCentrifugationDNA ExtractionReal-time PCRSelenite Crystal MediumColony SelectionAutomated Microbial IdentificationO-antigen CharacterizationH-antigen Characterization

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