Salmonella Shigella Screening
Salmonella Shigella Screening - Related Videos
06:55
A High-throughput Platform for the Screening of Salmonella spp./Shigella spp.
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2018
Salmonella spp./Shigella spp. are common pathogens attributed to diarrhea. Here, we describe a high-throughput platform for the screening of Salmonella spp./Shigella spp. using real-time PCR combined with guided culture.
02:51
Stress Granule Formation During Shigella Infection of Cancerous Epithelial Cells
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2026
Source: Vonaesch, P. et. al., Immunofluorescence Analysis of Stress Granule Formation After Bacterial Challenge of Mammalian Cells. J. Vis. Exp. (2017)This video demonstrates the infection of epithelial cells with the pathogenic bacterium Shigella flexneri and the resulting host cellular response. Bacterial effector proteins disrupt host translation, triggering the formation of membrane-less organelles called stress granules, which reflect the cell's adaptive response to infection.
04:22
Intracellular Replication Assay for Shigella in Human Colonic Epithelial Cells
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2026
Source: Poore, K., et al. Epithelial Cell Infection Analyses with Shigella. J. Vis. Exp. (2024)This video demonstrates an in vitro assay to assess Shigella invasion and intracellular replication in human colonic epithelial monolayers. It highlights steps for infection, antibiotic selection, host cell lysis, and recovery of intracellular bacteria for downstream analysis.
08:24
Loop-Mediated Isothermal Amplification for Screening Salmonella in Animal Food and Confirming Salmonella from Culture Isolation
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Cited by 12 •
2020
Loop-mediated isothermal amplification (LAMP) is an isothermal nucleic acid amplification test (iNAAT) that has attracted broad interest in the pathogen detection field. Here, we present a multi-laboratory-validated Salmonella LAMP protocol as a rapid, reliable, and robust method for screening Salmonella in animal food and confirming presumptive Salmonella from culture isolation.
09:01
Chronic Salmonella Infected Mouse Model
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Cited by 12 •
2010
Establish a chronic bacterial infected mouse model with persistent Salmonella typhimurium colonization in intestine for 27 weeks.
FAQs
Selective media suppress much of the accompanying flora, making suspected enteric pathogens easier to recover from a mixed clinical specimen. Differential media then reveal characteristic colony reactions that help distinguish potentially relevant organisms from other growth. Using both functions together improves the efficiency of initial examination and directs attention toward colonies requiring further confirmation.
Characteristic colony reactions provide an initial indication that an isolate may belong to Salmonella or Shigella, but they do not by themselves establish the organism’s identity. The workflow therefore treats these findings as presumptive and subjects selected isolates to biochemical or serological confirmation. This sequence reduces the risk of basing clinical or public health decisions on screening appearance alone.
Biochemical and serological confirmation are follow-up approaches applied to presumptive isolates after screening media have identified colonies of interest. Biochemical confirmation evaluates organism-associated reactions, whereas serological confirmation uses antigen-related reactivity to support identification. Their role is to strengthen the interpretation of preliminary colony findings before results inform diagnosis, surveillance, or infection-control actions.
Clinical specimens contain organisms beyond the suspected pathogens, and this accompanying flora can complicate recovery and interpretation. Selective media address that challenge by suppressing much of the unwanted growth, while differential reactions help reveal colonies with relevant characteristics. Consistent use of this strategy allows laboratories to process specimens more efficiently and focus confirmatory work on presumptive isolates.
The workflow begins with inoculation of a clinical specimen onto selective and differential media. Laboratory personnel then examine the resulting growth for characteristic colony reactions and identify presumptive isolates for additional testing. Biochemical or serological confirmation follows, producing information that can support diagnostic interpretation and determine whether broader infection-control or public health follow-up is warranted.
The approach is useful when laboratories need to investigate suspected bacterial gastroenteritis or enteric infection, clarify a clinical diagnosis, or examine possible transmission events. Confirmed identification can also support outbreak investigation, infection-control decisions, and public health surveillance. In these settings, the method connects individual specimen testing with recognition of broader patterns affecting groups or communities.
Within immunology and infection, the workflow supplies organism-identification data from clinical specimens rather than relying only on symptoms or broader clinical categories. Serological confirmation provides an antigen-related follow-up option, while surveillance results help reveal patterns of transmission. Together, these findings support interpretation of host-associated infection events and the public health response to enteric pathogens.