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A Net Mold-based Method of Scaffold-free Three-Dimensional Cardiac Tissue Creation
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Bioengineering
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JoVE Journal Bioengineering
A Net Mold-based Method of Scaffold-free Three-Dimensional Cardiac Tissue Creation

A Net Mold-based Method of Scaffold-free Three-Dimensional Cardiac Tissue Creation

DOI:
10.3791/58252-v

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06:57 min

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August 05, 2018

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, , , , , , , , ,
1Department of Cardiac Surgery,The First Hospital of Jilin University, 2Division of Cardiac Surgery,Johns Hopkins Hospital

Chapters

  • 00:00Title
  • 00:14Process Flow of Cardiac Tissue Creation by Novel Mold
  • 01:21Spheroids Creation by Hanging Drop Method
  • 03:26Seeding Spheroids into the Mold Cavity
  • 05:16Result: The 3D Cardiac Patch Contains of Stacked Spheroids with Functional Beating after Removing from Novel Mold
  • 05:58Conclusion

Summary

Automatic Translation

Automatic Translation

This protocol describes a net mold-based method to create three-dimensional scaffold-free cardiac tissues with satisfactory structural integrity and synchronous beating behavior.

Tags

Scaffold-freeSpheroidsHanging Drop SystemMold-based MethodCardiomyocytesFibroblastsEndothelial CellsReusable DeviceSpontaneous FormationMechanical Integrity

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