Complete and Partial Aortic Occlusion for the Treatment of Hemorrhagic Shock in Swine
August 24th, 2018
Here, we present a protocol demonstrating a hemorrhagic shock model in swine that uses aortic occlusion as a bridge to definitive care in trauma. This model has application in testing a wide range of surgical and pharmacological therapeutic strategies.
Overview
This protocol demonstrates a hemorrhagic shock model in swine using aortic occlusion to bridge to definitive trauma care. It is applicable for testing various surgical and pharmacological strategies.
Key Study Components
Area of Science
- Trauma care
- Hemorrhagic shock
- Surgical techniques
Background
- This method addresses non-compressible torso hemorrhage.
- It provides a clinically relevant large animal model.
- The physiology of swine closely mimics that of humans.
- Hemorrhagic shock is a leading cause of preventable death in trauma.
Purpose of Study
- To evaluate endovascular resuscitative strategies.
- To improve trauma care outcomes.
- To enhance understanding of hemorrhagic shock treatment.
Methods Used
- Use of a hind limb pinch to confirm sedation.
- Conventional laryngoscope with a lighted Miller blade.
- Careful advancement of the blade to visualize the larynx.
- Maintaining oral mucosa integrity during the procedure.
Main Results
- Successful demonstration of the hemorrhagic shock model.
- Validation of surgical techniques in a swine model.
- Insights into the physiology relevant to human trauma.
- Potential for testing various therapeutic strategies.
Conclusions
- This model is a valuable tool for trauma research.
- It aids in developing effective treatment strategies.
- Future studies can build on this protocol to enhance trauma care.
Frequently Asked Questions
Executive Industry Relevance
Large animal models of hemorrhagic shock using aortic occlusion provide a translational bridge for evaluating endovascular interventions in trauma. This approach enables mechanistic de-risking and predictive assessment of resuscitative strategies prior to clinical translation. The model supports portfolio decisions by clarifying physiological responses and intervention thresholds relevant to human trauma care.
Strategic Applications in Biopharma R&D
Early Discovery & Target Validation
- Enables interrogation of endovascular device mechanisms in a physiologically relevant system.
- Supports functional validation of intervention targets for hemorrhagic shock management.
- Facilitates predictive confidence in device performance and safety profiles.
Screening & Assay Development
- Provides a validated large animal platform for quantitative hemodynamic and physiological measurements.
- Enables reproducible assessment of intervention efficacy and safety endpoints.
- Supports standardization of critical care and resuscitation protocols for downstream studies.
Translational & Preclinical Research
- Aligns with disease-relevant models for translational biomarker and outcome evaluation.
- Ensures continuity from discovery-stage device concepts to preclinical validation in human-like physiology.
- De-risks advancement of endovascular technologies for trauma and surgical indications.
Pipeline & Workflow Integration
This swine hemorrhagic shock model positions endovascular device testing at the intersection of discovery biology and preclinical validation, supporting lead identification and mechanistic de-risking.
- Discovery Biology: Clarifies physiological impact and mechanistic action of aortic occlusion strategies.
- Screening: Delivers quantitative outputs for hemodynamic, metabolic, and resuscitation endpoints.
- Analytics: Enables statistical comparison of intervention groups and outcome measures.
- Translational Research: Provides continuity for biomarker and efficacy assessment in a human-relevant model.
- Enterprise Reuse: Establishes a reusable platform for iterative device and therapeutic evaluation.
Operational & Enterprise Impact
- Scientific Value: Increases predictive confidence and reduces mechanistic ambiguity for trauma interventions.
- Operational Value: Standardizes large animal workflows and quantitative monitoring for reproducibility.
- Strategic Value: Informs go/no-go decisions and reduces late-stage biological risk for device portfolios.
- Portfolio Impact: Supports risk-adjusted prioritization and advancement of trauma care technologies.
Implementation Considerations
- Requires surgical expertise and critical care physiological knowledge.
- Demands access to advanced instrumentation for hemodynamic and metabolic monitoring.
- Necessitates cross-team standardization of surgical and resuscitation protocols.
- Adaptation may be needed for different device types or intervention strategies.
- Practical limitations include procedural complexity and resource intensity inherent to large animal models.
Biopharma FAQs
Chapters in this video
0:04
Title
0:52
Intubation and Femoral Artery and Vein Cannulation
3:31
Carotid Artery and External Jugular Cannulation
4:52
Pulmonary Artery (PA) Catheter Insertion and Cystostomy Tube Placement
6:12
Hemmorrhage, Aortic Balloon Occlusion Catheter Inflation, and Whole-blood Resuscitation
7:31
Results: Hemodynamic, Systemic, and Physiologic Responses to Hemorrhage and Balloon Inflation
9:12
Conclusion
