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JoVE Journal
Bioengineering

This content is Open Access.

Isolation and Decellularization of a Whole Porcine Pancreas
 

Isolation and Decellularization of a Whole Porcine Pancreas

Article DOI: 10.3791/58302
October 10th, 2018

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Summary October 10th, 2018

Tissue engineering of the whole pancreas is a challenge because of its exocrine and endocrine functions. We show a method for the dissection of an intact porcine pancreas and the process of successful decellularization by perfusion of detergents Triton X-100, sodium deoxycholate, and deoxyribonuclease.

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