Induction of Atherosclerotic Plaques Through Activation of Mineralocorticoid Receptors in Apolipoprotein E-deficient Mice

9.8K views

Cited by 8

07:36 min

September 26th, 2018

10.3791/58303-v

September 26th, 2018

9.8K views

Vincenzo Marzolla¹ , Andrea Armani¹ , Caterina Mammi¹ , Alessandra Feraco¹ , Massimiliano Caprio¹^,²

¹Laboratory of Cardiovascular Endocrinology, IRCCS San Raffaele Pisana, Rome, Italy, ²Department of Human Sciences and Promotion of the Quality of Life, San Raffaele Roma Open University, Rome, Italy

We describe a protocol to induce atherosclerosis in the aortic root of ApoE^-/- mice fed with an atherogenic diet, through a continuous release of aldosterone. Methods to characterize plaque composition are also described.

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Title

0:45

Osmotic Pump Filling and Implantation

2:38

Heart Dissection

4:03

Aortic Root Sectioning and Oil Red O Neutral Fat Staining

5:47

Results: Evaluation of the Aortic Root Phenotype in Aldosterone-induced Atherosclerosis in Apolipoprotein-E Knockout (ApoE ^-/-) Mice

6:28

Conclusion

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