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Induction of Atherosclerotic Plaques Through Activation of Mineralocorticoid Receptors in Apolipoprotein E-deficient Mice
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JoVE Journal Medicine
Induction of Atherosclerotic Plaques Through Activation of Mineralocorticoid Receptors in Apolipoprotein E-deficient Mice

Induction of Atherosclerotic Plaques Through Activation of Mineralocorticoid Receptors in Apolipoprotein E-deficient Mice

DOI:
10.3791/58303-v

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07:36 min

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September 26, 2018

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, , , ,
1Laboratory of Cardiovascular Endocrinology,IRCCS San Raffaele Pisana, Rome, Italy, 2Department of Human Sciences and Promotion of the Quality of Life,San Raffaele Roma Open University, Rome, Italy

Chapters

  • 00:04Title
  • 00:45Osmotic Pump Filling and Implantation
  • 02:38Heart Dissection
  • 04:03Aortic Root Sectioning and Oil Red O Neutral Fat Staining
  • 05:47Results: Evaluation of the Aortic Root Phenotype in Aldosterone-induced Atherosclerosis in Apolipoprotein-E Knockout (ApoE -/-) Mice
  • 06:28Conclusion

Summary

Automatic Translation

Automatic Translation

We describe a protocol to induce atherosclerosis in the aortic root of ApoE-/- mice fed with an atherogenic diet, through a continuous release of aldosterone. Methods to characterize plaque composition are also described.

Tags

Keywords: AtherosclerosisApolipoprotein E-deficient MiceMineralocorticoid ReceptorsAldosteroneMini-pump ImplantationAtherosclerotic Plaque InductionPerfusion

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