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Fabricating a Kidney Cortex Extracellular Matrix-Derived Hydrogel
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Bioengineering
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JoVE Journal Bioengineering
Fabricating a Kidney Cortex Extracellular Matrix-Derived Hydrogel

Fabricating a Kidney Cortex Extracellular Matrix-Derived Hydrogel

DOI:
10.3791/58314-v

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08:23 min

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October 13, 2018

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, , ,
1Department of Bioengineering,University of Washington, 2Department of Bioengineering, Center for Cardiovascular Biology, Institute for Stem Cell and Regenerative Medicine,University of Washington, 3Department of Medicine, Kidney Research Institute,University of Washington

Chapters

  • 00:04Title
  • 00:25Preparation of Human Kidney Tissue
  • 01:39Isolation of Extracellular Matrix from Human Kidney
  • 03:16Fabrication of Hydrogel Stock Solution
  • 04:46Hydrogel Gelation
  • 06:15Results: Characteristics of Decellularized Tissue and Cell Growth in kECM
  • 07:44Conclusion

Summary

Automatic Translation

Automatic Translation

Here we present a protocol to fabricate a kidney cortex extracellular matrix-derived hydrogel to retain the native kidney extracellular matrix (ECM) structural and biochemical composition. The fabrication process and its applications are described. Finally, a perspective on using this hydrogel to support kidney-specific cellular and tissue regeneration and bioengineering is discussed.

Tags

Keywords: Kidney CortexExtracellular MatrixHydrogelBiomaterialsCell Matrix InteractionsSulfateSDS SolutionRenal CapsuleMedullary RegionDecellularizationCell StrainerBleachHomogenization

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