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Fabrication of Anisotropic Polymeric Artificial Antigen Presenting Cells for CD8+ T Cell Activation
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Fabrication of Anisotropic Polymeric Artificial Antigen Presenting Cells for CD8+ T Cell Activation

Fabrication of Anisotropic Polymeric Artificial Antigen Presenting Cells for CD8+ T Cell Activation

DOI:
10.3791/58332-v

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10:16 min

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October 12, 2018

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, , ,
1Biomedical Engineering, Translational Tissue Engineering Center, Institute for Nanobiotechnology,Johns Hopkins University School of Medicine, 2Biomedical Engineering, Translational Tissue Engineering Center, Institute for Nanobiotechnology, Ophthalmology, Oncology, Neurosurgery, Materials Science and Engineering, Chemical and Biomolecular Engineering, and the Bloomberg-Kimmel Institute for Cancer Immunotherapy,Johns Hopkins University School of Medicine

Chapters

  • 00:04Title
  • 00:49Tunable Spherical Poly(Lactic-Co-Glycolic Acid) (PLGA) Fabrication
  • 03:30Tunable Polymeric Particle Fabrication
  • 06:21Surface Protein Conjugation for Artificial Antigen Presenting Cell (aAPC) Creation
  • 08:01Results: Representative Spherical and Ellipsoidal Particle Characterization
  • 09:49Conclusion

Summary

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Here, we present a protocol to quickly and reproducibly generate biologically inspired, biodegradable articifical antigen presenting cells (aAPC) with tunable size, shape, and surface protein presentation for T cell expansion ex vivo or in vivo.

Tags

Anisotropic Polymeric Artificial Antigen Presenting CellsCD8+ T Cell ActivationParticle Physical PropertiesBiomaterial Immune Cell InteractionsBiomimetic ParticlesCancer ImmunotherapyInfectious DiseaseType 1 DiabetesPLGAPolyvinyl AlcoholMicro-particle SynthesisNano-particle Synthesis

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