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哺乳动物细胞血浆膜检测效率的高通量测量
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Immunology and Infection
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JoVE Journal Immunology and Infection
High-throughput Measurement of Plasma Membrane Resealing Efficiency in Mammalian Cells
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哺乳动物细胞血浆膜检测效率的高通量测量

DOI:
10.3791/58351-v

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10:07 min

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January 07, 2019

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, ,
1Department of Microbial Infection and Immunity,The Ohio State University, 2Department of Microbiology,The Ohio State University, 3Infectious Diseases Institute,The Ohio State University, 4Division of Biostatistics, College of Public Health,The Ohio State University

Chapters

  • 00:04Title
  • 00:31Cell Plating and Multi-plate Reader Imaging Cytometer Setup
  • 03:41High-throughput Fluorescence-based Assay and Cell Enumeration
  • 07:29Results: Representative Plasma Membrane Efficiency Resealing in Mammalian Cells
  • 09:15Conclusion

Summary

Automatic Translation

Automatic Translation

在这里, 我们描述了一种高通量荧光检测, 通过在活细胞中的荧光和成像分析测量质膜的重接受效率。该检测方法可用于筛选调节哺乳动物细胞质膜重密封的药物或靶向基因。

Tags

Plasma Membrane ResealingHigh-throughputHeLa Cells96-well PlateFluorescencePropidium IodideKinetic AssayPlate ReaderOptical ConfigurationRead ModeRead Type

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