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Monitoring Hippo Signaling Pathway Activity Using a Luciferase-based Large Tumor Suppressor (LATS) Biosensor
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Chapters
- 00:04Title
- 00:50Investigation of a Putative Regulator of Hippo Signaling Using the LATS-BS
- 04:07A Screen to Identify Hippo Pathway Regulators Using LATS-BS
- 05:50Results: LATS-BS Utilized to Measure Activation and Inhibition Effect of a Small Molecule on LATS Kinase Activity
- 06:22Conclusion
Here we present a luciferase-based biosensor to quantify the kinase activity of large tumor suppressor (LATS)-a central kinase in the Hippo signaling pathway. This biosensor has diverse applications in basic and translational research aimed at investigating Hippo pathway regulators in vitro and in vivo.
