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Fabricación de una cubierta de Nafion, reduce el óxido/polianilina Chemiresistive Sensor de pH Monitor en tiempo real durante la fermentación microbiana
JoVE Journal
Bioengineering
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JoVE Journal Bioengineering
Manufacturing of a Nafion-coated, Reduced Graphene Oxide/Polyaniline Chemiresistive Sensor to Monitor pH in Real-time During Microbial Fermentation
DOI:

11:18 min

January 07, 2019

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Chapters

  • 00:04Title
  • 00:52Preparation of Graphite Oxide
  • 02:11GO-deposited Electrode Preparation
  • 03:07Reduction of GO to Electrochemically Reduced Graphene Oxide
  • 04:29Polyaniline Functionalization of the ERGO Electrode
  • 06:09ERGO-PA Electrode Testing at Different pH (Pre-calibration Before Nafion Coating)
  • 06:46Preparation of the Nafion-coated ERGO-PA Electrode
  • 07:22Preparation of L. lactis Culture Medium
  • 08:23Testing of the ERGO-PA-NA pH Response in an L. lactis Fermentation Experiment
  • 09:08Results: Fabrication of ERGO-PA-NA Electrodes to Detect pH Changes During Microbial Fermentation
  • 10:41Conclusion

Summary

Automatic Translation

Aquí, Divulgamos el protocolo para la fabricación de un sensor de pH micro de chemiresistive de óxido grafeno recubierto de Nafion, polianilina funcionalizados, reducidos electroquímicamente. Este sensor de estado sólido, basada en chemiresistor pH micro puede detectar cambios de pH en tiempo real durante un proceso de fermentación de Lactococcus lactis .

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