Journal
/
Cancer Research
/
Assessment of the Metabolic Profile of Primary Leukemia Cells
JoVE Journal
Cancer Research
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Cancer Research
Assessment of the Metabolic Profile of Primary Leukemia Cells

Assessment of the Metabolic Profile of Primary Leukemia Cells

DOI:
10.3791/58426-v

•

06:21 min

•

November 21, 2018

•

,
1CLIP – Childhood Leukemia Investigation Prague, Second Faculty of Medicine, Laboratory of Molecular Genetics,Charles University, Prague

Chapters

  • 00:04Title
  • 00:28Bone Marrow Mononuclear Cell Isolation and Culture
  • 01:33Extracellular Flux Analysis Preparation
  • 02:41Cell Seeding and Sensor Cartridge Loading
  • 04:11Results: Representative Leukemic Blast Extracellular Flux Analysis
  • 05:54Conclusion

Summary

Automatic Translation

Automatic Translation

Here we present a protocol for the isolation of leukemic cells from leukemia patients bone marrow and analysis of their metabolic state. Assessment of the metabolic profile of primary leukemia cells could help to better characterize the demand of primary cells and could lead up to more personalized medicine.

Tags

Keywords: Primary Leukemia CellsMetabolic ProfileMetabolic PathwayExtracellular Flux AnalysisDensity Gradient CentrifugationMononuclear CellsCell AdhesiveExtracellular Flux AnalyzerRPMI MediumCell Counting

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/58426?article_type=v

A Detailed Protocol for Characterizing the Murine C1498 Cell Line and its Associated Leukemia Mouse Model
check_url/58426?article_type=v

Preparation and Metabolic Assay of 3-dimensional Spheroid Co-cultures of Pancreatic Cancer Cells and Fibroblasts
check_url/58426?article_type=v

Preparation of Primary Acute Lymphoblastic Leukemia Cells in Different Cell Cycle Phases by Centrifugal Elutriation
check_url/58426?article_type=v

Flow Cytometry to Estimate Leukemia Stem Cells in Primary Acute Myeloid Leukemia and in Patient-derived-xenografts, at Diagnosis and Follow Up
check_url/58426?article_type=v

Rapid Generation of Primary Murine Melanocyte and Fibroblast Cultures
check_url/58426?article_type=v

Flow Cytometric Analysis of Mitochondrial Reactive Oxygen Species in Murine Hematopoietic Stem and Progenitor Cells and MLL-AF9 Driven Leukemia
check_url/58426?article_type=v

Subcellular Fractionation of Primary Chronic Lymphocytic Leukemia Cells to Monitor Nuclear/Cytoplasmic Protein Trafficking
check_url/58426?article_type=v

Two Flow Cytometric Approaches of NKG2D Ligand Surface Detection to Distinguish Stem Cells from Bulk Subpopulations in Acute Myeloid Leukemia
check_url/58426?article_type=v

Preparation of Cytoplasmic and Nuclear Long RNAs from Primary and Cultured Cells
check_url/58426?article_type=v

Co-Culture and Transduction of Murine Thymocytes on Delta-Like 4-Expressing Stromal Cells to Study Oncogenes in T-Cell Leukemia

Read Article