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Generation of Cationic Nanoliposomes for the Efficient Delivery of In Vitro Transcribed Messenger RNA
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Bioengineering
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JoVE Journal Bioengineering
Generation of Cationic Nanoliposomes for the Efficient Delivery of In Vitro Transcribed Messenger RNA

Generation of Cationic Nanoliposomes for the Efficient Delivery of In Vitro Transcribed Messenger RNA

DOI:
10.3791/58444-v

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08:29 min

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February 01, 2019

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, , , , , , ,
1Department of Thoracic and Cardiovascular Surgery, Clinical Research Laboratory,University Medical Center, 2Atherothrombosis and Vascular Biology,Baker Heart & Diabetes Institute, 3Department of Medicine,Monash University

Chapters

  • 00:04Title
  • 00:48Generation of Cationic Nanoliposomes
  • 02:25Complexation of Synthetic mRNA
  • 03:03Preparation of the Cells for Transfection and Transfection of the Cells
  • 03:54Analysis of Cell Transfection Efficacy Using Flow Cytometry and Fluorescence Microscopy
  • 05:33Results: Analysis of Generated Cationic Nanoliposomes
  • 07:13Conclusion

Summary

Automatic Translation

Automatic Translation

Here we describe a protocol for the generation of cationic nanoliposomes, which is based on the dry-film method and can be used for the safe and efficient delivery of in vitro transcribed messenger RNA.

Tags

Cationic NanoliposomesMRNA DeliveryTransfectionNanoparticleLipid FilmExtrusionNanolipoplexA549 Cells

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