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Generation of Cationic Nanoliposomes for the Efficient Delivery of In Vitro Transcribed Messenger RNA
JoVE Journal
Bioengineering
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JoVE Journal Bioengineering
Generation of Cationic Nanoliposomes for the Efficient Delivery of In Vitro Transcribed Messenger RNA
DOI:

08:29 min

February 01, 2019

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Chapters

  • 00:04Title
  • 00:48Generation of Cationic Nanoliposomes
  • 02:25Complexation of Synthetic mRNA
  • 03:03Preparation of the Cells for Transfection and Transfection of the Cells
  • 03:54Analysis of Cell Transfection Efficacy Using Flow Cytometry and Fluorescence Microscopy
  • 05:33Results: Analysis of Generated Cationic Nanoliposomes
  • 07:13Conclusion

Summary

Automatic Translation

Here we describe a protocol for the generation of cationic nanoliposomes, which is based on the dry-film method and can be used for the safe and efficient delivery of in vitro transcribed messenger RNA.

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