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JoVE Journal Neuroscience
Forberedelse af akut rygmarven skiver til hele-celle Patch-klemme optagelse i Substantia Gelatinosa neuroner
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Forberedelse af akut rygmarven skiver til hele-celle Patch-klemme optagelse i Substantia Gelatinosa neuroner

Article DOI: 10.3791/58479-v • 08:30 min • January 18th, 2019
January 18th, 2019 •
1, 1, 2, 2, 2, 2, 2,3
1Department of Pain, First Affiliated Hospital of Nanchang University, 2Department of Pediatrics, First Affiliated Hospital of Nanchang University, 3Center for Laboratory Medicine, First Affiliated Hospital of Nanchang University

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Her beskriver vi de væsentlige skridt for hele-celle patch-klemme optagelser foretaget fra substantia gelatinosa (SG) neuroner i rygmarven skive i vitro . Denne metode giver den iboende membran egenskaber, synaptisk transmission og morfologiske karakterisering af SG neuroner som skal undersøges.

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Neurovidenskab spørgsmålet 143 neurovidenskab rygmarven skive substantia gelatinosa neuron hele-celle patch-clamp Elektrofysiologi morfologi in vitro
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