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利用斑马鱼对肠道转运时间进行高通量测量
JoVE Journal
Biology
This content is Free Access.
JoVE Journal
Biology
High-throughput Measurement of Gut Transit Time Using Larval Zebrafish
Please note that all translations are automatically generated.
Click here for the English version.
利用斑马鱼对肠道转运时间进行高通量测量
DOI:
10.3791/58497-v
•
06:48 min
•
October 23, 2018
•
Steven Cassar
,
Xin Huang
,
Todd Cole
1
Preclinical Safety
,
AbbVie
,
2
Statistics
,
AbbVie
,
3
Integrated Science and Technology
,
AbbVie
Chapters
00:04
Title
01:27
Training the Larvae to Feed Using Non-dyed Food
02:10
Preparing Fluorescent Food, Feeding the Larvae, and Applying Treatments
04:08
Measuring Initial and Subsequent Fluorescence from Each Well
05:04
Results: High-throughput Measurement of Gut Transit Time
06:22
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
本议定书的目的是通过高通量的方式, 测量荧光标记食物在斑马鱼肠道中的转运时间。
Tags
Gut Transit Time
Larval Zebrafish
High-throughput Measurement
Fluorescent Label
Fecal Matter
Plate Spectrophotometer
Gastrointestinal Biology
Gene Expression
Environmental Challenges
Gut Function
Traditional Methods
Feeding
Rinsing
Transfer
Fluorescent Food
Test Compound
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