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Neuroscience

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JoVE Journal Neuroscience
Kvantitativ Autoradiographic metode for fastsettelse av regionale priser på cerebral protein syntese in vivo
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Kvantitativ Autoradiographic metode for fastsettelse av regionale priser på cerebral protein syntese in vivo

Article DOI: 10.3791/58503-v • 11:01 min • June 28th, 2019
June 28th, 2019 •
1, 1, 1, 1, 1
1Section on Neuroadaptation and Protein Metabolism, National Institute of Mental Health, National Institutes of Health

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Proteinsyntese er en kritisk biologisk prosess for celler. I hjernen er det nødvendig for adaptive endringer. Måling av utbredelsen av proteinsyntese i intakt hjernen krever nøye metodisk betraktninger. Her presenterer vi L-[1-14C]-leucine kvantitative autoradiographic metode for bestemmelse av regionale utbredelsen av cerebral protein syntese in vivo.

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Nevrovitenskap proteinsyntese hjernen protein degradering autoradiografi oversettelse aminosyrer anisomycin
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